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Literature summary extracted from

  • Oda, K.; Sugitani, M.; Fukuhara, K.; Murao, S.
    Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative bacterium (1987), Biochim. Biophys. Acta, 923, 463-469.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.100 1,2-epoxy-3-(4-nitrophenoxy)propane modifies the enzyme with concomitant loss of activity Pseudomonas sp.
3.4.21.100 HgCl2 slight Pseudomonas sp.
3.4.21.100 additional information diazoacetyl-DL-norleucine methyl ester; diisopropyl fluorophosphate (1 mM); EDTA (1 mM); iodoacetic acid (1 mM); not: pepstatin; p-chloromercuribenzenesulfonic acid; phenyl methane sulfonyl fluoride (1 mM); Streptomyces pepsin inhibitor Pseudomonas sp.
3.4.21.100 PCMB slight Pseudomonas sp.
3.4.21.101 1,2-epoxy-3-(4-nitrophenoxy)propane
-
 Pseudomonas sp.
3.4.21.101 chymostatin weak Pseudomonas sp.
3.4.21.101 HgCl2 slight Pseudomonas sp.
3.4.21.101 additional information diisopropyl fluorophosphate; EDTA; iodoacetic acid; methyl 2-diazoacetamidohexanoate; not: pepstatin; p-chloromercuribenzenesulfonic acid; phenylmethanesulfonyl fluoride Pseudomonas sp.
3.4.21.101 PCMB slight Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.100 42000
-
 Pseudomonas sp., gel filtration Pseudomonas sp.
3.4.21.101 42000
-
 gel filtration Pseudomonas sp.
3.4.21.101 43000
-
 1 * 43000, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.100 Pseudomonas sp.
-
 No. 101
-
3.4.21.100 Pseudomonas sp. No. 101
-
 No. 101
-
3.4.21.101 Pseudomonas sp.
-
-
-
3.4.21.101 Pseudomonas sp. No. 101
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.100 additional information less than 0.05% carbohydrate Pseudomonas sp.
3.4.21.101 additional information contains less than 0.05% carbohydrate Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.100
-
 Pseudomonas sp.
3.4.21.101 strain No. 101 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.100 culture filtrate
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.100 additional information
-
-
 Pseudomonas sp.
3.4.21.101 additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.100 Acid-denatured hemoglobin + H2O
-
 Pseudomonas sp. ?
-
 ?
3.4.21.100 Acid-denatured hemoglobin + H2O
-
 Pseudomonas sp. No. 101 ?
-
 ?
3.4.21.100 casein + H2O
-
 Pseudomonas sp. ?
-
 ?
3.4.21.100 casein + H2O
-
 Pseudomonas sp. No. 101 ?
-
 ?
3.4.21.101 Acid-denatured hemoglobin + H2O
-
 Pseudomonas sp. ?
-
 ?
3.4.21.101 Acid-denatured hemoglobin + H2O
-
 Pseudomonas sp. No. 101 ?
-
 ?
3.4.21.101 casein + H2O
-
 Pseudomonas sp. ?
-
 ?
3.4.21.101 casein + H2O
-
 Pseudomonas sp. No. 101 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.100 monomer 1 * 42000, Pseudomonas sp., SDS-PAGE Pseudomonas sp.
3.4.21.101 monomer 1 * 43000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.100 50
-
-
 Pseudomonas sp.
3.4.21.101 50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.100 45
-
 15 min, stable below Pseudomonas sp.
3.4.21.100 65
-
 complete loss of activity after 15 min Pseudomonas sp.
3.4.21.101 37
-
 pH 4.0-5.5, 15 h, stable Pseudomonas sp.
3.4.21.101 45
-
 15 min, stable below Pseudomonas sp.
3.4.21.101 65
-
 15 min, complete loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.100 3
-
 acid-denatured hemoglobin, casein Pseudomonas sp.
3.4.21.101 3
-
 acid-denatured hemoglobin, casein Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.100 4 5.5 15 h, 37°C, stable Pseudomonas sp.
3.4.21.101 4 5.5 37°C, 15 h, stable Pseudomonas sp.
3.4.21.101 4.8
-
 15 min, stable below 50°C, complete loss of activity at 60°C Pseudomonas sp.