EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.13.2.1 | NAD+ | restores activity of enzyme treated with acid ammonium sulfate | Rattus norvegicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.13.2.1 | into Escherichia coli | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.13.2.1 | D244E | very bad binding capacity for NAD+ | Rattus norvegicus |
EC Number | General Stability | Organism |
---|---|---|
3.13.2.1 | wild-type and D244E rather resistant to inactivation by trypsin, wild-type resistant to chymotrypsin, D224E loses 63% of activity after treatment with chymotrypsin | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.13.2.1 | acid ammonium sulfate | - |
Rattus norvegicus | |
3.13.2.1 | S-adenosyl-L-homocysteine | competitive | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.13.2.1 | 0.00125 | - |
adenosine | wild-type | Rattus norvegicus | |
3.13.2.1 | 0.00143 | - |
adenosine | D244E | Rattus norvegicus | |
3.13.2.1 | 0.0115 | - |
S-adenosyl-L-homocysteine | D244E | Rattus norvegicus | |
3.13.2.1 | 0.0141 | - |
S-adenosyl-L-homocysteine | wild-type | Rattus norvegicus | |
3.13.2.1 | 0.0216 | - |
DL-homocysteine | D244E | Rattus norvegicus | |
3.13.2.1 | 0.0565 | - |
DL-homocysteine | wild-type | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.13.2.1 | 46000 | - |
wild-type and D244E, homotetramer, 4 * 46000, SDS-PAGE | Rattus norvegicus |
3.13.2.1 | 190000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Rattus norvegicus | - |
adenosine + L-homocysteine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.13.2.1 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.13.2.1 | mutant enzyme D244E, to homogeneity | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.13.2.1 | liver | - |
Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.13.2.1 | DL-homocysteine + adenosine | - |
Rattus norvegicus | S-adenosyl-DL-homocysteine | - |
r | |
3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Rattus norvegicus | adenosine + L-homocysteine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.13.2.1 | tetramer | wild-type and D244E, homotetramer, 4 * 46000, SDS-PAGE | Rattus norvegicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.13.2.1 | additional information | - |
wild-type in 70% formic acid for 24 h at 37°C: cleavage into 2 peptides of 26000 and 21000 Da | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
3.13.2.1 | NAD+ | - |
Rattus norvegicus |