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Literature summary extracted from

  • Thompson, J.; Robrish, S.A.; Bouma, C.L.; Freedberg, D.I.; Folk, J.E.
    Phospho-beta-glucosidase from Fusobacterium mortiferum: Purification, clonig, and inactivation by 6-phosphoglucono-sigma-lactone (1997), J. Bacteriol., 179, 1636-1645.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.86 D-glucono-delta-lactone
-
 Fusobacterium mortiferum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.86 0.0092
-
 4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate
-
 Fusobacterium mortiferum
3.2.1.86 0.0291
-
 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
 Fusobacterium mortiferum
3.2.1.86 0.0875
-
 laminaribiose 6-phosphate
-
 Fusobacterium mortiferum
3.2.1.86 0.0886
-
 Salicin 6-phosphate
-
 Fusobacterium mortiferum
3.2.1.86 0.0977
-
 arbutin 6-phosphate
-
 Fusobacterium mortiferum
3.2.1.86 0.1212
-
 sophorose 6-phosphate
-
 Fusobacterium mortiferum
3.2.1.86 0.1384
-
 methyl-beta-D-glucoside 6-phosphate
-
 Fusobacterium mortiferum
3.2.1.86 0.1823
-
 cellobiose 6-phosphate
-
 Fusobacterium mortiferum
3.2.1.86 0.3355
-
 gentiobiose 6-phosphate
-
 Fusobacterium mortiferum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.86 53000
-
 SDS-PAGE Fusobacterium mortiferum
3.2.1.86 53560
-
 MALDI-MS Fusobacterium mortiferum
3.2.1.86 54000
-
 gel filtration chromatography Fusobacterium mortiferum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.86 Fusobacterium mortiferum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.86 23 fold Fusobacterium mortiferum

Storage Stability

EC Number Storage Stability Organism
3.2.1.86 -20°C, 6 months, no significant decline in activity Fusobacterium mortiferum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.86 4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate + H2O
-
 Fusobacterium mortiferum 4-methylumbelliferone + D-glucose 6-phosphate
-
 ?
3.2.1.86 arbutin 6-phosphate + H2O
-
 Fusobacterium mortiferum hydroquinone + D-glucose 6-phosphate
-
 ?
3.2.1.86 cellobiose 6-phosphate + H2O
-
 Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.86 esculin 6-phosphate + H2O
-
 Fusobacterium mortiferum ?
-
 ?
3.2.1.86 gentiobiose 6-phosphate + H2O
-
 Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.86 laminaribiose 6-phosphate + H2O
-
 Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.86 methyl beta-D-glucoside monophosphate + H2O
-
 Fusobacterium mortiferum ?
-
 ?
3.2.1.86 methyl beta-glucoside 6-phosphate + H2O
-
 Fusobacterium mortiferum ?
-
 ?
3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
 Fusobacterium mortiferum p-nitrophenol + D-glucopyranose 6-phosphate
-
 ?
3.2.1.86 salicin 6-phosphate + H2O
-
 Fusobacterium mortiferum 2-hydroxymethylphenol + D-glucose 6-phosphate
-
 ?
3.2.1.86 sophorose 6-phosphate + H2O
-
 Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.86 35 40
-
 Fusobacterium mortiferum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.86 6.5 7
-
 Fusobacterium mortiferum