Any feedback?
Literature summary extracted from
- The agarase gene (dagA) of Streptomyces coelicolor A3(2): affinity purification and characterization of the cloned gene product (1987), J. Gen. Microbiol., 133, 2089-2096.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.81 | expression in Streptomyces lividans, natural Dag- | Streptomyces coelicolor |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.81 | Ca2+ | in higher concentrations, 10-20 mM | Streptomyces coelicolor |  |
| 3.2.1.81 | Mn2+ | in higher concentrations, 10-20 mM | Streptomyces coelicolor | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.81 | intracellular | - |
Streptomyces coelicolor | 5622 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.81 | agarose + H2O | Streptomyces coelicolor | - |
neoagarotetraose | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.81 | Streptomyces coelicolor | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.81 | proteolytic modification | - |
Streptomyces coelicolor |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.81 | - |
Streptomyces coelicolor |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.81 | 616 | - |
- |
Streptomyces coelicolor |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.81 | agarose + H2O | - |
Streptomyces coelicolor | neoagarotetraose | - |
ir | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.81 | 6 | - |
in imidazol or acetate buffers | Streptomyces coelicolor |
| 3.2.1.81 | 7.5 | - |
in Tris buffer, activity significant lower | Streptomyces coelicolor |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.81 | 5 | 8.5 | in imidazol or acetate buffers | Streptomyces coelicolor |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
