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Literature summary extracted from

  • Bom, I.J.; Dielbandhoesing, S.K.; Harvey, K.N.; Oomes, S.J.C.M.; Klis, F.M.; Brul, S.
    A new tool for studying the molecular architecture of the fungal cell wall: one-step purification of recombinant Trichoderma beta-(1-6)-glucanase expressed in Pichia pastoris (1998), Biochim. Biophys. Acta, 1425, 419-424.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.75 expression in Pichia pastoris Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.75 extracellular
-
 Trichoderma harzianum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.75 43000
-
 x * 43000, SDS-PAGE Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.75 Trichoderma harzianum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.75 154fold, 68fold expressed in Pichia pastoris Trichoderma harzianum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.75 culture filtrate
-
 Trichoderma harzianum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.75 204
-
-
 Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.75 pustulan + H2O
-
 Trichoderma harzianum glucose + gentiobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.75 ? x * 43000, SDS-PAGE Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.75 5
-
-
 Trichoderma harzianum