EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.33 | analysis | useful in structural determination of glycogen and amylopectin | Oryctolagus cuniculus |
3.2.1.33 | medicine | diagnosis, even prenatal, of type III glycogen storage disease: marked decrease or absence of amylo-1,6-glucosidase, glycogen debranching enzyme | Homo sapiens |
3.2.1.68 | analysis | - |
Pseudomonas amyloderamosa |
3.2.1.68 | analysis | structural determination of glycogen and starch components | Cytophaga sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | additional information | - |
- |
Saccharomyces cerevisiae |
3.2.1.33 | additional information | - |
- |
Oryctolagus cuniculus |
3.2.1.33 | 70000 | - |
glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea | Saccharomyces cerevisiae |
3.2.1.33 | 85000 | - |
glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea | Saccharomyces cerevisiae |
3.2.1.33 | 122000 | - |
glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea | Saccharomyces cerevisiae |
3.2.1.33 | 210000 | - |
glycogen debranching enzyme, gel filtration and PAGE | Saccharomyces cerevisiae |
3.2.1.33 | 267000 | 279000 | glycogen debranching enzyme, equilibrium ultracentrifugation | Oryctolagus cuniculus |
3.2.1.33 | 280000 | - |
- |
Saccharomyces cerevisiae |
3.2.1.33 | 280000 | - |
glycogen debranching enzyme, sedimentation equilibrium | Saccharomyces cerevisiae |
3.2.1.68 | 94000 | - |
- |
Pseudomonas amyloderamosa |
3.2.1.68 | 120000 | - |
- |
Cytophaga sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | Mammalia | phi-dextrin | ? | - |
? | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | Saccharomyces cerevisiae | phi-dextrin | ? | - |
? | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | Oryctolagus cuniculus | phi-dextrin | ? | - |
? | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | Oryctolagus cuniculus | total degradation of glycogen is accomplished by the combined action of glycogen phosphorylase and glycogen debranching enzyme | ? | - |
? | |
3.2.1.33 | additional information | Mammalia | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | ? | - |
? | |
3.2.1.33 | additional information | Saccharomyces cerevisiae | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | ? | - |
? | |
3.2.1.33 | additional information | Oryctolagus cuniculus | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | ? | - |
? | |
3.2.1.33 | additional information | Mammalia | glycogen debranching system, indirect debranching enzymes, is the dominant type of glycogen debranching mechanism, where glycogen is stored | ? | - |
? | |
3.2.1.68 | starch + H2O | Pseudomonas amyloderamosa | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.33 | Homo sapiens | - |
- |
- |
3.2.1.33 | Mammalia | - |
generally present in mammalian tissues | - |
3.2.1.33 | Oryctolagus cuniculus | - |
- |
- |
3.2.1.33 | Saccharomyces cerevisiae | - |
- |
- |
3.2.1.33 | Saccharomyces cerevisiae | - |
baker's yeast | - |
3.2.1.68 | Cytophaga sp. | - |
- |
- |
3.2.1.68 | Pseudomonas amyloderamosa | - |
gram-negative soil bacterium, strain SB-15 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.33 | glycogen debranching enzyme | Saccharomyces cerevisiae |
3.2.1.33 | glycogen debranching enzyme | Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.33 | erythrocyte | - |
Mammalia | - |
3.2.1.33 | leukocyte | - |
Mammalia | - |
3.2.1.33 | leukocyte | - |
Homo sapiens | - |
3.2.1.33 | liver | - |
Mammalia | - |
3.2.1.33 | liver | - |
Homo sapiens | - |
3.2.1.33 | liver | - |
Oryctolagus cuniculus | - |
3.2.1.33 | muscle | - |
Mammalia | - |
3.2.1.33 | muscle | - |
Homo sapiens | - |
3.2.1.33 | muscle | - |
Oryctolagus cuniculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | 4 | - |
substrate: glycogen phosphorylase-limit dextrin | Oryctolagus cuniculus |
3.2.1.33 | 6 | - |
- |
Saccharomyces cerevisiae |
3.2.1.33 | 7.6 | - |
- |
Oryctolagus cuniculus |
3.2.1.33 | 8.3 | - |
- |
Oryctolagus cuniculus |
3.2.1.33 | 8.4 | - |
- |
Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.33 | 2 D-glucose | - |
Oryctolagus cuniculus | 6-O-alpha-D-glucosyl-D-glucose + H2O | isomaltose | r | |
3.2.1.33 | 63-alpha-glucosyl maltotetraose + H2O | branched pentasaccharide "fast B5" | Mammalia | maltotetraose + D-glucose | - |
r | |
3.2.1.33 | 63-alpha-glucosyl maltotetraose + H2O | branched pentasaccharide "fast B5" | Oryctolagus cuniculus | maltotetraose + D-glucose | - |
r | |
3.2.1.33 | 63-alpha-maltotriosyl maltotetraose + H2O | branched heptasaccharide B7 | Mammalia | ? | - |
? | |
3.2.1.33 | 63-alpha-maltotriosyl maltotetraose + H2O | branched heptasaccharide B7 | Oryctolagus cuniculus | ? | - |
? | |
3.2.1.33 | alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Saccharomyces cerevisiae | cyclohexaamylose + D-glucose | - |
r | |
3.2.1.33 | alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Mammalia | cyclohexaamylose + D-glucose | alpha-Schardinger dextrin | r | |
3.2.1.33 | alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Saccharomyces cerevisiae | cyclohexaamylose + D-glucose | alpha-Schardinger dextrin | r | |
3.2.1.33 | alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Oryctolagus cuniculus | cyclohexaamylose + D-glucose | alpha-Schardinger dextrin | r | |
3.2.1.33 | alpha-dextrin + H2O | - |
Mammalia | alpha-dextrin + D-glucose | - |
? | |
3.2.1.33 | alpha-dextrin + H2O | - |
Saccharomyces cerevisiae | alpha-dextrin + D-glucose | - |
? | |
3.2.1.33 | amylopectin + H2O | very poor substrate | Oryctolagus cuniculus | amylopectin + D-glucose | partially debranched | ? | |
3.2.1.33 | amylopectin + H2O | slowly hydrolyzed | Mammalia | amylopectin + D-glucose | - |
? | |
3.2.1.33 | amylopectin + H2O | slowly hydrolyzed | Saccharomyces cerevisiae | amylopectin + D-glucose | - |
? | |
3.2.1.33 | amylopectin + H2O | slowly hydrolyzed | Saccharomyces cerevisiae | amylopectin + D-glucose | partially debranched | ? | |
3.2.1.33 | amylopectin + H2O | phi-dextrin, beta-dextrin and phi-beta-dextrin from amylopectin | Saccharomyces cerevisiae | amylopectin + D-glucose | partially debranched | ? | |
3.2.1.33 | amylopectin + H2O | phi-dextrin, beta-dextrin and phi-beta-dextrin from amylopectin | Oryctolagus cuniculus | amylopectin + D-glucose | partially debranched | ? | |
3.2.1.33 | amylose + H2O | - |
Saccharomyces cerevisiae | ? | - |
r | |
3.2.1.33 | amylose + H2O | - |
Oryctolagus cuniculus | ? | - |
r | |
3.2.1.33 | beta-amylase limit dextrin + H2O | beta-dextrin | Saccharomyces cerevisiae | limit dextrin + D-glucose | - |
r | |
3.2.1.33 | beta-amylase limit dextrin + H2O | beta-dextrin | Oryctolagus cuniculus | limit dextrin + D-glucose | - |
r | |
3.2.1.33 | D-glucose + maltooligosaccharide | - |
Saccharomyces cerevisiae | maltooligosaccharide | branched | r | |
3.2.1.33 | D-glucose + maltooligosaccharide | - |
Oryctolagus cuniculus | maltooligosaccharide | branched | r | |
3.2.1.33 | glycogen + H2O | rabbit liver glycogen: very poor substrate, shellfish glycogen: not a substrate | Oryctolagus cuniculus | glycogen + D-glucose | - |
r | |
3.2.1.33 | glycogen + H2O | native glycogen from shellfish and rabbit liver | Saccharomyces cerevisiae | glycogen + D-glucose | - |
r | |
3.2.1.33 | glycogen + H2O | reverse reaction: incorporation of glucose into glycogen | Mammalia | glycogen + D-glucose | - |
r | |
3.2.1.33 | glycogen + H2O | reverse reaction: incorporation of glucose into glycogen | Homo sapiens | glycogen + D-glucose | - |
r | |
3.2.1.33 | glycogen + H2O | reverse reaction: incorporation of glucose into glycogen | Saccharomyces cerevisiae | glycogen + D-glucose | - |
r | |
3.2.1.33 | glycogen + H2O | reverse reaction: incorporation of glucose into glycogen | Oryctolagus cuniculus | glycogen + D-glucose | - |
r | |
3.2.1.33 | glycogen + H2O | rabbit liver glycogen: slowly | Oryctolagus cuniculus | glycogen + D-glucose | - |
r | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | best substrate | Mammalia | limit dextrin + D-glucose | - |
r | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | best substrate | Saccharomyces cerevisiae | limit dextrin + D-glucose | - |
r | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | best substrate | Oryctolagus cuniculus | limit dextrin + D-glucose | partially debranched glycogen | r | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Mammalia | limit dextrin + D-glucose | - |
r | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Saccharomyces cerevisiae | limit dextrin + D-glucose | - |
r | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Oryctolagus cuniculus | limit dextrin + D-glucose | partially debranched glycogen | r | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Mammalia | ? | - |
? | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Saccharomyces cerevisiae | ? | - |
? | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Oryctolagus cuniculus | ? | - |
? | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | total degradation of glycogen is accomplished by the combined action of glycogen phosphorylase and glycogen debranching enzyme | Oryctolagus cuniculus | ? | - |
? | |
3.2.1.33 | additional information | substrate specificities | Mammalia | ? | - |
? | |
3.2.1.33 | additional information | substrate specificities | Saccharomyces cerevisiae | ? | - |
? | |
3.2.1.33 | additional information | substrate specificities | Oryctolagus cuniculus | ? | - |
? | |
3.2.1.33 | additional information | 63-alpha-glucosyl maltotriose: not a substrate | Oryctolagus cuniculus | ? | - |
? | |
3.2.1.33 | additional information | panose: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
3.2.1.33 | additional information | pullulan: not a substrate | Mammalia | ? | - |
? | |
3.2.1.33 | additional information | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | Mammalia | ? | - |
? | |
3.2.1.33 | additional information | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | Saccharomyces cerevisiae | ? | - |
? | |
3.2.1.33 | additional information | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | Oryctolagus cuniculus | ? | - |
? | |
3.2.1.33 | additional information | glycogen debranching system, indirect debranching enzymes, is the dominant type of glycogen debranching mechanism, where glycogen is stored | Mammalia | ? | - |
? | |
3.2.1.33 | phosphorylase beta-amylase limit dextrin + H2O | limit dextrin, beta-phi-dextrin, derived from the action of beta-amylase on the phosphorylase limit dextrin | Saccharomyces cerevisiae | limit dextrin + D-glucose | - |
r | |
3.2.1.33 | phosphorylase beta-amylase limit dextrin + H2O | limit dextrin, beta-phi-dextrin, derived from the action of beta-amylase on the phosphorylase limit dextrin | Oryctolagus cuniculus | limit dextrin + D-glucose | - |
r | |
3.2.1.68 | amylopectin + H2O | waxy maize amylopectin, potato amylopectin, amylopectin beta-dextrin | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
3.2.1.68 | amylopectin + H2O | or the beta-limit dextrins, cleaves the branching points completely in vitro | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
3.2.1.68 | amylopectin phi-dextrin + H2O | substrate on which all 3 types of debranching enzymes act | Pseudomonas amyloderamosa | maltotetraose | - |
? | |
3.2.1.68 | glycogen + H2O | cleaves the branching points completely in vitro | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
3.2.1.68 | glycogen + H2O | oyster glycogen, glycogen beta-dextrin, rabbit liver glycogen | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
3.2.1.68 | glycogen + H2O | or the beta-limit dextrins | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
3.2.1.68 | additional information | direct debranching enzyme, attacks unmodified glycogen and/or amylopectin with hydrolysis of the 1,6-bond, smallest substrate is not known | Pseudomonas amyloderamosa | ? | - |
? | |
3.2.1.68 | starch + H2O | - |
Pseudomonas amyloderamosa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.33 | More | evidence for a subunit of 120000 | Oryctolagus cuniculus |
3.2.1.33 | trimer | - |
Saccharomyces cerevisiae |
3.2.1.33 | trimer | glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea | Saccharomyces cerevisiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.33 | glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Mammalia |
3.2.1.33 | glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Homo sapiens |
3.2.1.33 | glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Saccharomyces cerevisiae |
3.2.1.33 | glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Oryctolagus cuniculus |
3.2.1.68 | Cythophaga isoamylase | - |
Cytophaga sp. |
3.2.1.68 | plant isoamylase | - |
- |
3.2.1.68 | yeast isoamylase | - |
- |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | additional information | - |
pH-optimum depends on type of buffer | Oryctolagus cuniculus |
3.2.1.33 | additional information | - |
pH-optimum depends on substrate and type of buffer, overview | Oryctolagus cuniculus |
3.2.1.33 | 6 | 6.6 | - |
Saccharomyces cerevisiae |
3.2.1.33 | 6.6 | - |
anionic buffer, substrate: glycogen phosphorylase limit dextrin | Oryctolagus cuniculus |
3.2.1.33 | 7.2 | - |
cationic buffer, substrate: glycogen phosphorylase limit dextrin | Oryctolagus cuniculus |