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Literature summary extracted from

  • Mapes, C.A.; Suelter, C.H.; Sweeley, C.C.
    Isolation and characterization of ceramide trihexosidases (form A) from human plasma (1973), J. Biol. Chem., 248, 2471-2479.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.22 digalactosylceramide in absence of sodium taurocholate and sodium chloride at low substrate concentrations Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ceramide lactoside
-
 Homo sapiens
3.2.1.22 ceramide trihexoside
-
 Homo sapiens
3.2.1.22 digalactosylceramide in presence of optimal sodium taurocholate and sodium chloride or at high substrate concentrations Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 24000
-
 4 * 24000, SDS-PAGE Homo sapiens
3.2.1.22 95000
-
 sucrose density gradient centrifugation, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.22 D-galactosyl-D-galactosyl-D-glucosyl-N-acylsphingosine + H2O Homo sapiens
-
 lactosyl-N-acylsphingosine + D-galactose
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.22 Butanol A1 retains enzymatic activity in 100% butanol, A2 retains activity in 20% butanol Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Homo sapiens
-
 2 forms, non-interconvertible: form A-1, form A-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 0.0396
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 D-galactosyl-D-galactosyl-D-glucosyl-N-acylsphingosine + H2O
-
 Homo sapiens lactosyl-N-acylsphingosine + D-galactose
-
 ?
3.2.1.22 additional information p-nitrophenyl-alpha-galactoside Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 tetramer 4 * 24000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 100
-
 A-1 75% loss of activity after boiling, A-2 100% loss of activity after boiling Homo sapiens