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Literature summary extracted from

  • Noronha, E.F.; Ulhoa, C.J.
    Purification and characterization of an endo-beta-1,3-glucanase from Trichoderma harzianum (1996), Can. J. Microbiol., 42, 1039-1044.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.39 Cu2+ 21% inhibition at 2 mM Trichoderma harzianum
3.2.1.39 Hg2+ strong inhibition at 2 mM Trichoderma harzianum
3.2.1.39 SDS 43% inhibition at 2 mM Trichoderma harzianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 Laminarin value in mg/ml Trichoderma harzianum
3.2.1.39 additional information
-
 Laminarin 1.18 Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.39 extracellular
-
 Trichoderma harzianum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.39 Ca2+ slight activation at 2 mM Trichoderma harzianum
3.2.1.39 Zn2+ slight activation at 2 mM Trichoderma harzianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 36000
-
 SDS-PAGE Trichoderma harzianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 cell wall glucan + H2O Trichoderma harzianum from different fungi, containing beta-1,3-, beta-1,4 and/or beta-1,6-linkages reducing sugar + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Trichoderma harzianum
-
 enzyme synthesis induced by growing on chitin-containing medium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, 2step chromatography Trichoderma harzianum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 culture filtrate
-
 Trichoderma harzianum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 711
-
-
 Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 beta-glucan + H2O beta-1,3- and beta-1,4-linkages Trichoderma harzianum ?
-
 ?
3.2.1.39 cell wall glucan + H2O from different fungi, containing beta-1,3-, beta-1,4 and/or beta-1,6-linkages Trichoderma harzianum reducing sugar + ?
-
 ?
3.2.1.39 laminarin + H2O only active against glucans containing beta-1,3-linkages Trichoderma harzianum D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 endo-beta-1,3-glucanase
-
 Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 45
-
-
 Trichoderma harzianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 45
-
 stable for 90 min Trichoderma harzianum
3.2.1.39 50
-
 50% activity Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 4.4
-
-
 Trichoderma harzianum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 2.6
-
 60% remaining activity Trichoderma harzianum
3.2.1.39 6.2
-
 60% remaining activity Trichoderma harzianum