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Literature summary extracted from

  • Fontaine, T.; Hartland, R.P.; Beauvais, A.; Diaquin, M.; Latge, J.P.
    Purification and characterization of an endo-1,3-beta-glucanase from Aspergillus fumigatus (1997), Eur. J. Biochem., 243, 315-321.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.39 Hg2+ inhibition at 0.4 mM Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 reduced curdlan value in mg/ml: 1.18 Aspergillus fumigatus
3.2.1.39 additional information
-
 reduced laminarin value in mg/ml: 0.3 Aspergillus fumigatus
3.2.1.39 additional information
-
 reduced laminarioctaose value in mg/ml: 0.3 Aspergillus fumigatus
3.2.1.39 additional information
-
 reduced laminaripentaose value in mg/ml: 0.3 Aspergillus fumigatus
3.2.1.39 additional information
-
 reduced lamanrihexaose value in mg/ml: 0.2 Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.39 cell wall
-
 Aspergillus fumigatus 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 74000
-
 SDS-PAGE, gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 alkali soluble 1,3-beta-glucan + H2O Aspergillus fumigatus produced by solubilised 1,3-beta-glucan synthase complex of A. fumigatus reducing sugar + ?
-
 ?
3.2.1.39 cell wall glucan + H2O Aspergillus fumigatus putative role during cell wall morphogenesis reducing sugar + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Aspergillus fumigatus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.39 glycoprotein glycosylated with mannose residues, only O-linked Aspergillus fumigatus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, 4step chroamtography Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O low activity Aspergillus fumigatus ?
-
 ?
3.2.1.39 3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O laminaritetraose Aspergillus fumigatus ?
-
 ?
3.2.1.39 alkali soluble 1,3-beta-glucan + H2O produced by solubilised 1,3-beta-glucan synthase complex of A. fumigatus Aspergillus fumigatus reducing sugar + ?
-
 ?
3.2.1.39 cell wall glucan + H2O putative role during cell wall morphogenesis Aspergillus fumigatus reducing sugar + ?
-
 ?
3.2.1.39 curdlan + H2O insoluble reduced 1,3-beta-glucan Aspergillus fumigatus ?
-
 ?
3.2.1.39 laminarin + H2O soluble reduced 1,3-beta-glucan, release of reducing 1,3-beta-oligosaccharides of variable size, presence of a single 1,6-beta branched glucose every three glucose units of a linear 1,3-beta-glucan chain prevents hydrolysis Aspergillus fumigatus D-glucose + ?
-
 ?
3.2.1.39 reduced laminarioligosaccharides + H2O velocity of catalytic reaction increases with the number of 1,3-beta-glucosidic bonds, preferentially cleaves between the glucose residues at positions 2 and 3 from the reducing end Aspergillus fumigatus laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 monomer 1 * 74000, SDS-PAGE, gel filtration Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 60
-
-
 Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 55
-
 stable up to, activity lost at 70°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 7
-
-
 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 8 10 stable within Aspergillus fumigatus