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Literature summary extracted from
- Studies on sphingomyelinase of Bacillus cereus. I. Purification and properties (1978), Biochim. Biophys. Acta, 528, 247-256.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | deoxycholate | 3fold stimulation at concentrations above 0.1% | Bacillus cereus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Ca2+ | completely inhibited at 5 mM CaCl2 | Bacillus cereus | |
| 3.1.4.12 | EDTA | complete inhibition at 0.25 mM | Bacillus cereus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.12 | extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Ca2+ | partly restores the EDTA-inhibited enzyme activity | Bacillus cereus | |
| 3.1.4.12 | Mg2+ | causes basal activation, reverses EDTA inhibition | Bacillus cereus | |
| 3.1.4.12 | Zn2+ | partly restores the EDTA-inhibited enzyme activity | Bacillus cereus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 24000 | - |
gel filtration | Bacillus cereus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | Bacillus cereus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Bacillus cereus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | 980fold | Bacillus cereus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | culture medium | - |
Bacillus cereus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | additional information | - |
different assay conditions, substrates etc. | Bacillus cereus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.4.12 | 4°C for 24 h, at pH 6-7.5 | Bacillus cereus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | lysophosphatidylcholine + H2O | - |
Bacillus cereus | 1-acylglycerol + choline phosphate | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | - |
Bacillus cereus | N-acylsphingosine + choline phosphate | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | - |
Bacillus cereus | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 37 | - |
- |
Bacillus cereus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 6 | 7 | - |
Bacillus cereus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 6 | 7.5 | - |
Bacillus cereus |
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