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Literature summary extracted from

  • Joyce, B.K.; Grisolia, S.
    Purification and properties of a nonspecific acid phosphatase from wheat germ (1960), J. Biol. Chem., 235, 2278-2280.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Ag+
-
 Triticum aestivum
3.1.3.2 Cu2+
-
 Triticum aestivum
3.1.3.2 Pb2+
-
 Triticum aestivum
3.1.3.2 phosphate in presence of Mg2+ Triticum aestivum
3.1.3.2 U6+
-
 Triticum aestivum
3.1.3.2 Zn2+
-
 Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.27
-
 3-phosphoglycerate
-
 Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Mg2+ stimulates Triticum aestivum
3.1.3.2 Mn2+ stimulates slightly Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 additional information
-
-
 Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-glycerate phosphate + H2O
-
 Triticum aestivum glycerate + phosphate
-
 ?
3.1.3.2 2,3-diphosphoglycerate + H2O
-
 Triticum aestivum ?
-
 ?
3.1.3.2 2-glycerophosphate + H2O
-
 Triticum aestivum glycerol + phosphate
-
 ?
3.1.3.2 3-phosphoglycerate + H2O
-
 Triticum aestivum glycerate + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Triticum aestivum AMP + phosphate
-
 ?
3.1.3.2 AMP + H2O
-
 Triticum aestivum adenosine + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Triticum aestivum ADP + phosphate
-
 ?
3.1.3.2 D-fructose 1,6-diphosphate + H2O
-
 Triticum aestivum ?
-
 ?
3.1.3.2 glucose 2-phosphate + H2O
-
 Triticum aestivum glucose + phosphate
-
 ?
3.1.3.2 glucose 3-phosphate + H2O
-
 Triticum aestivum glucose + phosphate
-
 ?
3.1.3.2 Glucose 6-phosphate + H2O
-
 Triticum aestivum Glucose + phosphate
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O
-
 Triticum aestivum pyruvate + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 50
-
 5 min, 76% loss of activity of partially purified enzyme Triticum aestivum
3.1.3.2 60
-
 5 min, 96% loss of activity partially purified enzyme Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.1
-
 hydrolysis of 3-phosphoglycerate or 2,3-diphosphoglycerate Triticum aestivum
3.1.3.2 5.7
-
-
 Triticum aestivum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 4 7.8 pH 4.0: about 35% of maximal activity, pH 7.8: about 20% of maximal activity Triticum aestivum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.2 4
-
 10 min, 20% loss of activity of partially purified enzyme Triticum aestivum
3.1.3.2 4.5
-
 10 min, 5% loss of activity of partially purified enzyme Triticum aestivum
3.1.3.2 5
-
 10 min, 4% loss of partially purified enzyme Triticum aestivum