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Literature summary extracted from

  • Imada, A.; Hunt, J.W.; van de Sande, H.; Sinskey, A.J.; Tannenbaum, S.R.
    Purification and properties of an intracellular ribonuclease from Candida lipolytica (1975), Biochim. Biophys. Acta, 395, 490-500.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.19 Cu2+
-
 Yarrowia lipolytica
4.6.1.19 Zn2+
-
 Yarrowia lipolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.19 32000
-
 gel filtration Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.19 Yarrowia lipolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.19
-
 Yarrowia lipolytica

Reaction

EC Number Reaction Comment Organism Reaction ID
4.6.1.19 RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] similar enzyme with possilbly exonucleolytic mechanism Yarrowia lipolytica
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.19 additional information
-
-
 Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.19 additional information not: DNA Yarrowia lipolytica ?
-
 ?
4.6.1.19 RNA + H2O tRNA Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
4.6.1.19 RNA + H2O rRNA Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
4.6.1.19 RNA + H2O polyguanylic acid and duplexes of synthetic homopolymers less sensitive Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
4.6.1.19 RNA + H2O polyuridylic acid Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
4.6.1.19 RNA + H2O polyadenylic acid Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
4.6.1.19 RNA + H2O preference for pC-purine bonds Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
4.6.1.19 RNA + H2O polycytidylic acid Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.19 60
-
-
 Yarrowia lipolytica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.19 50
-
 neutral pH, up to 50°C Yarrowia lipolytica
4.6.1.19 64
-
 30 min, pH 4.2: 95% inactivation, pH 6.6: 49% inactivation, pH 10.0: 64% inactivation Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.19 6
-
-
 Yarrowia lipolytica