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Literature summary extracted from

  • Guerrier-Takada, C.; Haydock, K.; Allen, L.; Altman, S.
    Metal ion requirements and other aspects of the reaction catalyzed by M1 RNA, the RNA subunit of ribonuclease P from Escherichia coli (1986), Biochemistry, 25, 1509-1515.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.26.5 2-methyl-2,4-pentanediol enhances cleavage reaction of the M1 RNA subunit alone Escherichia coli
3.1.26.5 Polyethylene glycol enhances cleavage reaction of the M1 RNA subunit alone Escherichia coli
3.1.26.5 spermidine M1 RNA subunit can catalyze the cleavage of tRNA precursor molecules in the presence of 5 mM spermine or spermidine Escherichia coli
3.1.26.5 spermine M1 RNA subunit can catalyze the cleavage of tRNA precursor molecules in the presence of 5 mM spermine or spermidine Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.5 Ca2+ Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations Escherichia coli
3.1.26.5 Mg2+ the only metal ion that can act as cofactor for activity of M1 RNA Escherichia coli
3.1.26.5 Mg2+ Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations Escherichia coli
3.1.26.5 Mn2+ Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations Escherichia coli
3.1.26.5 NH4+ stimulates activity of M1 RNA Escherichia coli
3.1.26.5 Sr2+ Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.5 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.5 pre-tRNA precursor + H2O
-
 Escherichia coli tRNA + 5'-oligoribonucleotide
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.5 5.5 9.5
-
 Escherichia coli