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Literature summary extracted from
- Biological catalysis by RNA (1986), Annu. Rev. Biochem., 55, 599-629.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.26.5 | Ca2+, Sr2+ and Mg2+ maintain the structure of the enzyme in solution | Escherichia coli |
| 3.1.26.5 | spermidine and spermine maintain the structure of the enzyme in solution | Escherichia coli |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.5 | Mg2+ | strict requirement for a divalent cation, Mg2+ or Mn2+. Hexacoordinated Mg2+ binds to the catalytic site on M1 RNA | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 3.1.26.5 | Mg2+ | strict requirement for a divalent cation, Mg2+ or Mn2+. Hexacoordinated Mg2+ binds to the catalytic site on M1 RNA | Bacillus subtilis | |
| 3.1.26.5 | Mg2+ | strict requirement for a divalent cation, Mg2+ or Mn2+. Hexacoordinated Mg2+ binds to the catalytic site on M1 RNA | Escherichia coli | |
| 3.1.26.5 | Mn2+ | strict requirement for a divalent cation, Mg2+ or Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.1.26.5 | Mn2+ | strict requirement for a divalent cation, Mg2+ or Mn2+ | Bacillus subtilis | |
| 3.1.26.5 | Mn2+ | strict requirement for a divalent cation, Mg2+ or Mn2+ | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.5 | pre-tRNA + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | tRNA + 5'-oligoribonucleotide | - |
? | |
| 3.1.26.5 | pre-tRNA + H2O | Bacillus subtilis | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | tRNA + 5'-oligoribonucleotide | - |
? | |
| 3.1.26.5 | pre-tRNA + H2O | Escherichia coli | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | tRNA + 5'-oligoribonucleotide | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.5 | Bacillus subtilis | - |
- |
- |
| 3.1.26.5 | Escherichia coli | - |
- |
- |
| 3.1.26.5 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.26.5 | ribonucleoprotein | the active site resides within the RNA moiety. The protein has a role in electrostatic shielding and aids in an essential conformational change | Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.1.26.5 | ribonucleoprotein | the active site resides within the RNA moiety. The protein has a role in electrostatic shielding and aids in an essential conformational change | Bacillus subtilis |
| 3.1.26.5 | ribonucleoprotein | the active site resides within the RNA moiety. The protein has a role in electrostatic shielding and aids in an essential conformational change | Escherichia coli |
| 3.1.26.5 | ribonucleoprotein | the enzyme requires an RNA as well as a protein subunit for its in vivo activity | Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.1.26.5 | ribonucleoprotein | the enzyme requires an RNA as well as a protein subunit for its in vivo activity | Bacillus subtilis |
| 3.1.26.5 | ribonucleoprotein | the enzyme requires an RNA as well as a protein subunit for its in vivo activity | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.5 | pre-tRNA + H2O | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | Salmonella enterica subsp. enterica serovar Typhimurium | tRNA + 5'-oligoribonucleotide | - |
? | |
| 3.1.26.5 | pre-tRNA + H2O | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | Bacillus subtilis | tRNA + 5'-oligoribonucleotide | - |
? | |
| 3.1.26.5 | pre-tRNA + H2O | the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules | Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? | |
| 3.1.26.5 | pre-tRNA precursor + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | tRNA + 5'-oligoribonucleotide | - |
? | |
| 3.1.26.5 | pre-tRNA precursor + H2O | - |
Bacillus subtilis | tRNA + 5'-oligoribonucleotide | - |
? | |
| 3.1.26.5 | pre-tRNA precursor + H2O | - |
Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.5 | 5.5 | 9.5 | - |
Escherichia coli |
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