Literature summary extracted from
Hsiao, Y.M.; Ho, H.C.; Wang, W.Y.; Tam, M.F.; Liao, T.H.
Purification and characterization of tilapia (Oreochromis mossambicus) deoxyribonuclease I. Primary structure and cDNA sequence (1997), Eur. J. Biochem., 249, 786-791.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.21.1 |
deducing amino acid composition from cDNA |
Oreochromis mossambicus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.21.1 |
iodoacetate |
50% inhibition at 0.1 M in presence of 4 mM CuCl2 after 15 min |
Oreochromis mossambicus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.21.1 |
Mg2+ |
highest activity at 10 mM, doubled activity upon addition of 1 mM Ca2+ |
Oreochromis mossambicus |
|
3.1.21.1 |
Mn2+ |
highest activity at 1 mM, addition of Ca2+ doesn't change activity |
Oreochromis mossambicus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.21.1 |
30000 |
- |
gel filtration, SDS-PAGE, thin-layer IEF, MS analysis |
Oreochromis mossambicus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.21.1 |
double-stranded DNA + H2O |
Oreochromis mossambicus |
- |
5'-phosphooligonucleotides + ? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.21.1 |
Oreochromis mossambicus |
- |
tilapia fish |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.1.21.1 |
glycoprotein |
carbohydrate-chain at Asn 18 |
Oreochromis mossambicus |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.21.1 |
to homogeneity by 4step chromatography |
Oreochromis mossambicus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.1.21.1 |
hepatopancreas |
- |
Oreochromis mossambicus |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.21.1 |
additional information |
- |
units defined as absorbance at 260 nm/ml assay solution |
Oreochromis mossambicus |
3.1.21.1 |
additional information |
- |
670 units/mg protein |
Oreochromis mossambicus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.21.1 |
double-stranded DNA + H2O |
- |
Oreochromis mossambicus |
5'-phosphooligonucleotides + ? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.21.1 |
monomer |
1 * 30000, SDS-PAGE, gel filtration |
Oreochromis mossambicus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.1.21.1 |
55 |
- |
inactivation after 10 min incubation |
Oreochromis mossambicus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.21.1 |
8.5 |
- |
in the presence of Mg2+ |
Oreochromis mossambicus |