Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rice, S.Q.J.; Southan, C.; Boyd, H.F.; Terrett, J.A.; MacPhee, C.H.; Moores, K.; Gloger, I.S.; Tew, D.G.
    Expression, purification and characterization of a human serine-dependent phospholipase A2 with high specificity for oxidized phospholipids and platelet activating factor (1998), Biochem. J., 330, 1309-1315.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.4 serine human serine dependent PLA2 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 into baculovirus, transfection of SF9 insect cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 3,4-dichloroisocoumarin
-
 Homo sapiens
3.1.1.4 Diethyl p-nitrophenyl phosphate
-
 Homo sapiens
3.1.1.4 diisopropyl fluorophosphate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 0.01
-
 1-decanoyl-2-(4-nitrophenylglutaryl) phosphate
-
 Homo sapiens
3.1.1.4 0.042
-
 platelet activating factor
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 cytoplasm
-
 Homo sapiens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 44160
-
 SDS-PAGE and liquid chromatography Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phospholipids + H2O Homo sapiens influence on platelet aggregation ?
-
 ?
3.1.1.4 platelet activating factor + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 brain
-
 Homo sapiens
-
3.1.1.4 leukocyte peripheral blood Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 35
-
 HSD-PLA2, 21fold purification, platelet activating factor for substrate Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-decanoyl-2-(4-nitrophenylglutaryl)glycerol 3-phosphate + H2O HSD-PLA2 Homo sapiens ?
-
 ?
3.1.1.4 oxidized phosphatidylcholines + H2O HSD-PLA2 Homo sapiens 1-acylglycerophosphocholine + oxidized fatty acids
-
 ?
3.1.1.4 phospholipids + H2O influence on platelet aggregation Homo sapiens ?
-
 ?
3.1.1.4 platelet activating factor + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.4 platelet activating factor + H2O PAF i.e. platelet activating factor, HSD-PLA2 Homo sapiens ?
-
 ?