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Literature summary extracted from

  • Pratviel-Sosa, F.; Mengin-Lecreulx, D.; van Heijenoort, J.
    Over-production, purification and properties of the uridine diphosphate N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli (1991), Eur. J. Biochem., 202, 1169-1176.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.9 potassium phosphate highly dependent on, optimal concentration: 11-16 mM, inhibition above 20 mM, phosphate ion is responsible for inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.9 0.0075
-
UDP-N-acetylmuramoyl-L-Ala
-
Escherichia coli
6.3.2.9 0.055
-
D-Glu
-
Escherichia coli
6.3.2.9 0.138
-
ATP
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.9 Mg2+ highly dependent on, optimal concentration: 5 mM Escherichia coli
6.3.2.9 potassium phosphate highly dependent on, optimal concentration: 11-16 mM, inhibition above 20 mM Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.9 Escherichia coli
-
K12 Km83, an overproducing strain that harbours recombinant plasmids bearing the murD gene under the control of the lac Pg promoter, JM83(pDML13) and JM83(pMLD50)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.9
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.9 0.261
-
Escherichia coli JM83(pDML13) Escherichia coli
6.3.2.9 1.92
-
Escherichia coli JM83 (pMLD50) Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.9 ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate ATP in form of MgATP2- Escherichia coli ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
-
?
6.3.2.9 ATP + UDP-N-acetylmuramoyl-L-Ala + Glu
-
Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.9 additional information
-
additional information
-
Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.9 8.9 9.2
-
Escherichia coli