EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.8 | 2-mercaptoethanol | stimulates | Staphylococcus aureus | |
6.3.2.8 | 2-mercaptoethanol | maximal concentration 2.5 mM | Staphylococcus aureus |
EC Number | General Stability | Organism |
---|---|---|
6.3.2.8 | decrease in stability at high salt concentrations is prevented by addition of 10% glycerol and 2.5 mM 2-mercaptoethanol | Staphylococcus aureus |
6.3.2.8 | relatively stable at low ionic strength, unstable at high concentrations of salt | Staphylococcus aureus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.8 | Ba2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
6.3.2.8 | Ca2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
6.3.2.8 | Cd2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
6.3.2.8 | Co2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
6.3.2.8 | Fe2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
6.3.2.8 | Hg2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
6.3.2.8 | Mg2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
6.3.2.8 | Ni2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
6.3.2.8 | Zn2+ | inhibits activation by Mn2+ | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.2.8 | 0.051 | - |
UDP-N-acetylmuramate | - |
Staphylococcus aureus | |
6.3.2.8 | 0.15 | - |
L-Ala | - |
Staphylococcus aureus | |
6.3.2.8 | 29 | - |
Gly | - |
Staphylococcus aureus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.8 | K+ | monovalent cation required, NH4+ or K+ | Staphylococcus aureus | |
6.3.2.8 | Mg2+ | divalent cation required, Mg2+ or Mn2+ | Staphylococcus aureus | |
6.3.2.8 | Mn2+ | divalent cation required | Staphylococcus aureus | |
6.3.2.8 | Mn2+ | Mg2+ or Mn2+, maximal activity at concentrations of Mn2+ equimolar to those of ATP | Staphylococcus aureus | |
6.3.2.8 | NH4+ | monovalent cation required, NH4+ or K+ | Staphylococcus aureus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.2.8 | 40000 | - |
gel filtration in absence and in presence of 2.5 mM 2-mercaptoethanol | Staphylococcus aureus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.2.8 | Staphylococcus aureus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.2.8 | - |
Staphylococcus aureus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.2.8 | 0.14 | - |
- |
Staphylococcus aureus |
EC Number | Storage Stability | Organism |
---|---|---|
6.3.2.8 | -18°C, 5 mM potassium phosphate, 10-20% loss of activity after 3 months | Staphylococcus aureus |
6.3.2.8 | 4°C, 5 mM potassium phosphate, overnight, 10-20% loss of activity. At 4°C, 250 mM potassium phosphate, 12 h, 30% loss of activity | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.8 | ATP + UDP-N-acetylmuramate + DL-Ser | - |
Staphylococcus aureus | ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser | - |
? | |
6.3.2.8 | ATP + UDP-N-acetylmuramate + Gly | - |
Staphylococcus aureus | ADP + phosphate + UDP-N-acetylmuramoyl-Gly | - |
? | |
6.3.2.8 | ATP + UDP-N-acetylmuramate + L-Ala | not active with D-Ala | Staphylococcus aureus | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? | |
6.3.2.8 | UTP + UDP-N-acetylmuramate + L-Ala | about 11% of the activity relative to ATP | Staphylococcus aureus | UDP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.2.8 | 50 | - |
5 mM potassium phosphate, pH 7.2, 20 min, 10-20% loss of activity. 60-90% loss of activity in 250 mM potassium phosphate buffer | Staphylococcus aureus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.2.8 | 7.8 | 8.5 | - |
Staphylococcus aureus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.3.2.8 | 7.5 | 9 | pH 7.5: about 60% of maximal activity, pH 9.0: about 70% of maximal activity | Staphylococcus aureus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
6.3.2.8 | 7.2 | - |
5 mM potassium phosphate, 50°C, 20 min, 10-20% loss of activity. 60-90% loss of activity in 250 mM potassium phosphate buffer | Staphylococcus aureus |