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Literature summary extracted from

  • Anwar, R.A.; Vlaovic, M.
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine synthetase from Bacillus sphaericus: activation by potassium phosphate (1986), Biochem. Cell Biol., 64, 297-303.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.7 0.37
-
 Lys in presence of 30 mM K2HPO4 Lysinibacillus sphaericus
6.3.2.7 0.42
-
 Lys in presence of 2 mM K2HPO4 Lysinibacillus sphaericus
6.3.2.7 0.44
-
 Lys in presence of 60 mM KCl Lysinibacillus sphaericus
6.3.2.7 0.49
-
 Lys in presence of 60 mM KCl Lysinibacillus sphaericus
6.3.2.7 0.52
-
 ATP in presence of 2 mM K2HPO4 Lysinibacillus sphaericus
6.3.2.7 0.53
-
 UDP-N-acetylmuramoyl-L-Ala-D-Glu in presence of 30 mM K2HPO4 Lysinibacillus sphaericus
6.3.2.7 0.55
-
 ATP in presence of 30 mM K2HPO4 Lysinibacillus sphaericus
6.3.2.7 0.57
-
 UDP-N-acetylmuramoyl-L-Ala-D-Glu in presence of 2 mM K2HPO4 Lysinibacillus sphaericus
6.3.2.7 0.59
-
 UDP-N-acetylmuramoyl-L-Ala-D-Glu in presence of 60 mM KCl Lysinibacillus sphaericus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.7 Cl- activation by different anions in decreasing order: HPO42-, Cl-, SO42- , potassium salts give higher activity than the corresponding sodium salts Lysinibacillus sphaericus
6.3.2.7 HPO42- activation by different anions in decreasing order: HPO42-, Cl-, SO42- , potassium salts give higher activity than the corresponding sodium salts Lysinibacillus sphaericus
6.3.2.7 Mg2+ required Lysinibacillus sphaericus
6.3.2.7 Mg2+ optimal concentration: 10 mM Lysinibacillus sphaericus
6.3.2.7 phosphate the presence of phosphate changes the enzyme conformation to a catalytically more active form Lysinibacillus sphaericus
6.3.2.7 SO42- activation by different anions in decreasing order: HPO42-, Cl-, SO42- , potassium salts give higher activity than the corresponding sodium salts Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.7 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys Lysinibacillus sphaericus one step in the biosynthesis of bacterial cell walls ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.7 Lysinibacillus sphaericus
-
 enzyme is synthesized during vegetative growth
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.7
-
 Lysinibacillus sphaericus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.7 vegetative
-
 Lysinibacillus sphaericus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.7 0.245
-
-
 Lysinibacillus sphaericus

Storage Stability

EC Number Storage Stability Organism
6.3.2.7 -20°C, stable for over 3 months when stored in the lyophilized form Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.7 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys
-
 Lysinibacillus sphaericus ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys
-
 ?
6.3.2.7 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys one step in the biosynthesis of bacterial cell walls Lysinibacillus sphaericus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.7 8.5
-
-
 Lysinibacillus sphaericus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.7 8 8.5 activity decreases sharply below pH 8.0, optimal activity at pH 8.5 Lysinibacillus sphaericus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.2.7 7.5
-
 rapid loss of activity at or below, even at 0°C Lysinibacillus sphaericus
6.3.2.7 8
-
 stable at Lysinibacillus sphaericus