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Literature summary extracted from

  • Meyer, E.; Leonard, N.J.; Bhat, B.; Stubbe, J.; Smith, J.M.
    Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway (1992), Biochemistry, 31, 5022-5032.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.21
-
 Escherichia coli
6.3.2.6 SAICAR synthetase is cloned into a lambdaPL expression vector to give plasmid pJS408, overexpression Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.6 0.036
-
 Asp
-
 Escherichia coli
6.3.2.6 0.04
-
 MgATP2-
-
 Escherichia coli
6.3.2.6 1.3
-
 1-(5'-phosphoribosyl)-5-amino-4-carboxyimidazole
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.21 17000
-
 x * 17000, purE encoded protein, + x * 39000, purK encoded protein, SDS-PAGE. PurE has an octameric MW of 126000 by gel filtration. PurK has a dimeric MW of 79000 by gel filtration Escherichia coli
4.1.1.21 39000
-
 x * 17000, purE encoded protein, + x * 39000, purK encoded protein, SDS-PAGE. PurE has an octameric MW of 126000 by gel filtration. PurK has a dimeric MW of 79000 by gel filtration Escherichia coli
5.4.99.18 17000
-
 8 * 17000, SDS-PAGE Escherichia coli
5.4.99.18 126000
-
 gel filtration Escherichia coli
5.4.99.18 136000
-
 sucrose density gradient ultracentrifugation Escherichia coli
6.3.2.6 27000
-
 3 * 27000, SDS-PAGE Escherichia coli
6.3.2.6 76000
-
 ultracentrifugation Escherichia coli
6.3.4.18 39000
-
 2 * 39000, SDS-PAGE Escherichia coli
6.3.4.18 78000
-
 sucrose density gradient ultracentrifugation Escherichia coli
6.3.4.18 79000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.21 1-(5-Phosphoribosyl)-5-aminoimidazole + CO2 Escherichia coli sixth step in the purine biosynthetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.21 Escherichia coli
-
-
-
5.4.99.18 Escherichia coli
-
-
-
6.3.2.6 Escherichia coli
-
-
-
6.3.4.18 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.21
-
 Escherichia coli
5.4.99.18
-
 Escherichia coli
6.3.2.6
-
 Escherichia coli
6.3.4.18
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.21 additional information
-
-
 Escherichia coli
5.4.99.18 additional information
-
-
 Escherichia coli
6.3.2.6 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.21 1-(5-Phosphoribosyl)-5-aminoimidazole + CO2
-
 Escherichia coli 1-(5-Phosphoribosyl)-5-amino-4-imidazolecarboxylate
-
 ?
4.1.1.21 1-(5-Phosphoribosyl)-5-aminoimidazole + CO2 sixth step in the purine biosynthetic pathway Escherichia coli ?
-
 ?
6.3.2.6 ATP + 1-(5'-phosphoribosyl)-5-amino-4-carboxyimidazole + L-Asp
-
 Escherichia coli ADP + phosphate + 1-(5'-phosphoribosyl)-5-amino-4-(N-succinocarboxamide)-imidazole
-
 ?
6.3.2.6 ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate ATP in form of MgATP2- Escherichia coli ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.21 ? x * 17000, purE encoded protein, + x * 39000, purK encoded protein, SDS-PAGE. PurE has an octameric MW of 126000 by gel filtration. PurK has a dimeric MW of 79000 by gel filtration Escherichia coli
5.4.99.18 octamer 8 * 17000, SDS-PAGE Escherichia coli
6.3.2.6 trimer 3 * 27000, SDS-PAGE Escherichia coli
6.3.4.18 dimer 2 * 39000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
5.4.99.18 PurE
-
 Escherichia coli
6.3.4.18 PurK
-
 Escherichia coli