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Literature summary extracted from

  • Ebbole, D.J.; Zalkin, H.
    Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis (1987), J. Biol. Chem., 262, 8274-8287.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.10
-
 Bacillus subtilis
4.1.1.21
-
 Bacillus subtilis
4.3.2.2
-
 Bacillus subtilis
6.3.2.6 cloning of a 12-gene cluster encoding 9 enzymes for de novo purine nucleotide synthesis Bacillus subtilis
6.3.3.1 cloning and characterization of a 12-gene cluster encoding nine enzymes for de novo purine nucleotide synthesis. The cluster is likely an operon and is organized into three groups of overlapping genes followed by the last gene: purEKB-purC(or)QLF-purMNH(J)-purD Bacillus subtilis
6.3.4.13 12-gene cluster encoding nine enzymes for de novo purine nucleotide synthesis Bacillus subtilis
6.3.5.3 purL gene encoded aminator subunit, purQ gene encoded glutamine amide transfer subunit Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.10 55746
-
 2 * 55746, open reading frame Bacillus subtilis
4.1.1.21 17176
-
 x * 17176, subunit I, + x * 42150, subunit II, calculation from nucleotide sequence Bacillus subtilis
4.1.1.21 42150
-
 x * 17176, subunit I, + x * 42150, subunit II, calculation from nucleotide sequence Bacillus subtilis
4.3.2.2 49490
-
 x * 49490, calculation from nucleotide sequence Bacillus subtilis
6.3.2.6 27431
-
 x * 27431, calculation from nucleotide sequence Bacillus subtilis
6.3.3.1 37057
-
 x * 37057, calculation from nucleotide sequence Bacillus subtilis
6.3.4.13 45253
-
 x * 45253, calculation from nucleotide sequence Bacillus subtilis
6.3.5.3 24755
-
 x * 24755, glutamine amide transfer subunit, + x * 80300, aminator subunit Bacillus subtilis
6.3.5.3 80300
-
 x * 24755, glutamine amide transfer subunit, + x * 80300, aminator subunit Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.10 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide Bacillus subtilis de novo purine synthesis step 10 ?
-
 ?
6.3.2.6 ATP + 1-(5'-phosphoribosyl)-5-amino-4-carboxyimidazole + L-Asp Bacillus subtilis one of the enzymes of the series involved in the biosynthesis of the purine nucleotides de novo ?
-
 ?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln Bacillus subtilis enzyme of de novo purine nucleotide synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.10 Bacillus subtilis
-
-
-
4.1.1.21 Bacillus subtilis
-
-
-
4.3.2.2 Bacillus subtilis
-
-
-
6.3.2.6 Bacillus subtilis
-
-
-
6.3.3.1 Bacillus subtilis
-
-
-
6.3.4.13 Bacillus subtilis
-
-
-
6.3.5.3 Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.10 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide de novo purine synthesis step 10 Bacillus subtilis ?
-
 ?
4.1.1.21 1-(5-Phosphoribosyl)-5-aminoimidazole + CO2
-
 Bacillus subtilis 1-(5-Phosphoribosyl)-5-amino-4-imidazolecarboxylate
-
 ?
6.3.2.6 ATP + 1-(5'-phosphoribosyl)-5-amino-4-carboxyimidazole + L-Asp
-
 Bacillus subtilis ADP + phosphate + 1-(5'-phosphoribosyl)-5-amino-4-(N-succinocarboxamide)-imidazole
-
 ?
6.3.2.6 ATP + 1-(5'-phosphoribosyl)-5-amino-4-carboxyimidazole + L-Asp one of the enzymes of the series involved in the biosynthesis of the purine nucleotides de novo Bacillus subtilis ?
-
 ?
6.3.3.1 ATP + 2-(formamido)-N1-(5-phosphoribosyl)acetamidine
-
 Bacillus subtilis ADP + phosphate + 1-(5-phosphoribosyl)-5-aminoimidazole
-
 ?
6.3.4.13 ATP + 5-phosphoribosylamine + Gly
-
 Bacillus subtilis ADP + phosphate + 5-phosphoribosylglycinamide
-
 ?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln enzyme of de novo purine nucleotide synthesis Bacillus subtilis ?
-
 ?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
 Bacillus subtilis ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.10 dimer 2 * 55746, open reading frame Bacillus subtilis
4.1.1.21 ? x * 17176, subunit I, + x * 42150, subunit II, calculation from nucleotide sequence Bacillus subtilis
4.3.2.2 ? x * 49490, calculation from nucleotide sequence Bacillus subtilis
6.3.2.6 ? x * 27431, calculation from nucleotide sequence Bacillus subtilis
6.3.3.1 ? x * 37057, calculation from nucleotide sequence Bacillus subtilis
6.3.4.13 ? x * 45253, calculation from nucleotide sequence Bacillus subtilis
6.3.5.3 ? x * 24755, glutamine amide transfer subunit, + x * 80300, aminator subunit Bacillus subtilis