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Literature summary extracted from

  • Tambe, S.M.; Kaklij, G.S.; Kelkar, S.M.; Parekh, L.J.
    Two distinct molecular forms of phytase from Klebsiella aerogenes: evidence for unusually small active enzyme peptide (1994), J. Ferment. Bioeng., 77, 23-27.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.062
-
 myo-inositol hexakisphosphate high molecular weight form Klebsiella aerogenes
3.1.3.8 0.114
-
 myo-inositol hexakisphosphate low molecular weight form Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 12800
-
 low molecular weight form, gel filtration Klebsiella aerogenes
3.1.3.8 700000
-
 high molecular weight form, gel filtration Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Klebsiella aerogenes
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Klebsiella aerogenes
-
 a low molecular weight form and a high molecular weight form
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Klebsiella aerogenes 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 additional information
-
 thermal stability of the high molecular weight form and the low molecular weight form Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 4.5
-
 high molecular weight form Klebsiella aerogenes
3.1.3.8 5.2
-
 low molecular weight form Klebsiella aerogenes