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Literature summary extracted from

  • Kerovuo, J.; Lauraeus, M.; Nurminen, P.; Kalkkinen, N.; Apajalahti, J.
    Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis (1998), Appl. Environ. Microbiol., 64, 2079-2085.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8
-
 Bacillus subtilis

General Stability

EC Number General Stability Organism
3.1.3.8 Ca2+ is required for stability Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 EDTA
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 Ca2+ required Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 43000
-
 x * 43000, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Bacillus subtilis
-
-
-
3.1.3.8 Bacillus subtilis VTT E-68013
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 29
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 ADP + H2O 75% of the activity with myo-inositol hexakisphosphate Bacillus subtilis ?
-
 ?
3.1.3.8 ADP + H2O 75% of the activity with myo-inositol hexakisphosphate Bacillus subtilis VTT E-68013 ?
-
 ?
3.1.3.8 ATP + H2O 50% of the activity with myo-inositol hexakisphosphate Bacillus subtilis ?
-
 ?
3.1.3.8 ATP + H2O 50% of the activity with myo-inositol hexakisphosphate Bacillus subtilis VTT E-68013 ?
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Bacillus subtilis 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Bacillus subtilis VTT E-68013 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 43000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 55
-
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 7
-
 at 55°C Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 5.5 7.5 pH 5.5: about 35% of maximal activity, pH 7.5: about 95% of maximal activity Bacillus subtilis