Protein Variants | Comment | Organism |
---|---|---|
E599Q | 3000fold reduction of ATPase activity compared to wild-type | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
ATP | pH 9.0, 30°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Saccharomyces cerevisiae |
66000 | - |
gel filtration in presence of MgATP2- and BeFx or orthovanadate | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
recombinant protein expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant protein expressed in Escherichia coli | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + peptide/out | ATP best substrate | Saccharomyces cerevisiae | ADP + phosphate + peptide/in | - |
? | |
CTP + H2O + peptide/out | affinity for CTP is about 30fold lower compared to ATP | Saccharomyces cerevisiae | CDP + phosphate + peptide/in | - |
? | |
GTP + H2O + peptide/out | affinity for GTP is about 30fold lower compared to ATP | Saccharomyces cerevisiae | GDP + phosphate + peptide/in | - |
? | |
UTP + H2O + peptide/out | affinity for UTP is about 30fold lower compared to ATP | Saccharomyces cerevisiae | UDP + phosphate + peptide/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Mdl1 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
ATP | pH 9.0, 30°C | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Saccharomyces cerevisiae |