Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | in response to cadmium, yeast cells rapidly enhance expression of Pca1 by a post-transcriptional mechanism | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
Pca1 expression analysis | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the BY4741 haploid strain and null mutants. Fusion of the metal-responding degradation signal encompassing amino acids 250-350 to a stable protein demonstrates that it functions autonomously in a metal-responsive manner. Pca1 is not stabilized in a strain defective in endocytosis. Mutation of all seven cysteine residues to alanine alters the regulation of the enzyme expression, overview | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
Pca1 is a short-lived proteinwith t1?2 below 5 min and is subject to ubiquitination when cells are growing in media lacking cadmium | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Pca1 degradation is dependent on the proteasome but not vacuolar proteases | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | Pca1 contains a cytosolic cysteine-rich N-terminal extension of nearly 400 residues | Saccharomyces cerevisiae | - |
- |
plasma membrane | in the absence of cadmium, Pca1 is targeted for degradation before reaching the plasma membrane | Saccharomyces cerevisiae | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | Pca1, a P1B-type ATPase, plays a critical role in cadmium resistance in Saccharomyces cerevisiae by extruding intracellular cadmium as a cadmium-specific efflux pump, regulation requires cysteine residues within the cytosolic domain. Mechanistic insights into the cadmium-dependent control of Pca1 expression, pathway for Pca1 turnover and the mechanism of cadmium sensing that leads to up-regulation of Pca1, overview. Pca1 degradation is dependent on the proteasome but not vacuolar proteases | ? | - |
? | |
additional information | Saccharomyces cerevisiae BY4741 | Pca1, a P1B-type ATPase, plays a critical role in cadmium resistance in Saccharomyces cerevisiae by extruding intracellular cadmium as a cadmium-specific efflux pump, regulation requires cysteine residues within the cytosolic domain. Mechanistic insights into the cadmium-dependent control of Pca1 expression, pathway for Pca1 turnover and the mechanism of cadmium sensing that leads to up-regulation of Pca1, overview. Pca1 degradation is dependent on the proteasome but not vacuolar proteases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | cadmium sensing by cysteine residues within the domain, the N-terminal metal-responding degradation signal encompassing amino acids 250-350, circumvents ubiquitination and degradation of Pca1 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | Pca1 is a cadmium transporter, the N-terminal metal-responding degradation signal encompassing amino acids 250-350 functions autonomously in a metal-responsive manner, overview | Saccharomyces cerevisiae | ADP + phosphate + Cd2+/out | - |
? | |
ATP + H2O + Cd2+/in | Pca1 is a cadmium transporter, the N-terminal metal-responding degradation signal encompassing amino acids 250-350 functions autonomously in a metal-responsive manner, overview | Saccharomyces cerevisiae BY4741 | ADP + phosphate + Cd2+/out | - |
? | |
additional information | Pca1, a P1B-type ATPase, plays a critical role in cadmium resistance in Saccharomyces cerevisiae by extruding intracellular cadmium as a cadmium-specific efflux pump, regulation requires cysteine residues within the cytosolic domain. Mechanistic insights into the cadmium-dependent control of Pca1 expression, pathway for Pca1 turnover and the mechanism of cadmium sensing that leads to up-regulation of Pca1, overview. Pca1 degradation is dependent on the proteasome but not vacuolar proteases | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Pca1, a P1B-type ATPase, plays a critical role in cadmium resistance in Saccharomyces cerevisiae by extruding intracellular cadmium as a cadmium-specific efflux pump, regulation requires cysteine residues within the cytosolic domain. Mechanistic insights into the cadmium-dependent control of Pca1 expression, pathway for Pca1 turnover and the mechanism of cadmium sensing that leads to up-regulation of Pca1, overview. Pca1 degradation is dependent on the proteasome but not vacuolar proteases | Saccharomyces cerevisiae BY4741 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Pca1 possesses a metal-responding degradation signal encompassing amino acids 250-350, which functions autonomously in a metal-responsive manner, being part of the cytosolic cysteine-rich N-terminal extension of nearly 400 residues | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
cadmium-transporting P1B-type ATPase | - |
Saccharomyces cerevisiae |
PCA1 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |