Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | complete inhibition of Na+ transport at 0.5 mM | Acetobacterium woodii | |
Ag+ | complete inhibition of Na+ transport at 0.1 mM | Acetobacterium woodii | |
Cu2+ | complete inhibition of Na+ transport at 0.08 mM | Acetobacterium woodii | |
diphenyleniodonium chloride | complete inhibition of Na+ transport at 0.04 mM | Acetobacterium woodii | |
diphenyliodonium chloride | complete inhibition of Na+ transport at 0.04 mM | Acetobacterium woodii | |
additional information | the enzyme complex is inhibited by sodium ionophores but not protonophores, demonstrating a direct coupling of the ferredoxin:NAD+ oxidoreductase activity to Na+ transport | Acetobacterium woodii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
Na+/in | pH 6.0, 30°C | Acetobacterium woodii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Acetobacterium woodii | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | Acetobacterium woodii | electron transfer from reduced ferredoxin to NAD+ as acceptor drives the generation of a transmembrane ion gradient | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacterium woodii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | - |
Acetobacterium woodii | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? | |
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | electron transfer from reduced ferredoxin to NAD+ as acceptor drives the generation of a transmembrane ion gradient | Acetobacterium woodii | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Na+-translocating ferredoxin:NAD+ oxidoreductase | - |
Acetobacterium woodii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acetobacterium woodii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Acetobacterium woodii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | iron–sulfur and flavin-containing electron transport complex | Acetobacterium woodii | |
iron-sulfur centre | iron–sulfur and flavin-containing electron transport complex | Acetobacterium woodii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000035 | - |
pH 6.0, 30°C, inhibition of ferredoxin:NAD+ oxidoreductase activity | Acetobacterium woodii | diphenyleniodonium chloride | |
0.001 | - |
pH 6.0, 30°C, inhibition of ferredoxin:NAD+ oxidoreductase activity | Acetobacterium woodii | diphenyliodonium chloride | |
0.00175 | - |
pH 6.0, 30°C, inhibition of ferredoxin:NAD+ oxidoreductase activity | Acetobacterium woodii | Cu2+ | |
0.00375 | - |
pH 6.0, 30°C, inhibition of ferredoxin:NAD+ oxidoreductase activity | Acetobacterium woodii | Ag+ | |
0.155 | - |
pH 6.0, 30°C, inhibition of ferredoxin:NAD+ oxidoreductase activity | Acetobacterium woodii | 1,10-phenanthroline |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme complex is involved in Na+-motive, anaerobic respiration with caffeate as electron acceptor | Acetobacterium woodii |