Cloned (Comment) | Organism |
---|---|
wild type and mutant enzymes expressed in Escherichia coli JM109 | Bacillus sp. PS3 |
Protein Variants | Comment | Organism |
---|---|---|
P100A | activity almost completely abolished, trimer, heat labile | Bacillus sp. PS3 |
P104A | activity almost completely abolished, trimer, heat labile | Bacillus sp. PS3 |
P116A | large decrease in activity, almost fully active at pH 10 | Bacillus sp. PS3 |
P146A | activity almost completely abolished, trimer, heat labile | Bacillus sp. PS3 |
P14A | large decrease in activity, formation of trimers during heat treatment | Bacillus sp. PS3 |
P39A | slight increase in activity, almost fully active at pH 10 | Bacillus sp. PS3 |
P43A | large decrease in activity, formation of trimers during heat treatment | Bacillus sp. PS3 |
P59A | large decrease in activity, narrow pH optimum at pH 8 | Bacillus sp. PS3 |
P69A | slight decrease in activity | Bacillus sp. PS3 |
P72A | activity completely abolished, monomer | Bacillus sp. PS3 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
diphosphate | P69A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
0.47 | - |
diphosphate | wild type enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
0.55 | - |
diphosphate | P146A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
0.6 | - |
diphosphate | P39A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
0.64 | - |
diphosphate | P116A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
0.82 | - |
diphosphate | P14A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
0.88 | - |
diphosphate | P100A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
0.96 | - |
diphosphate | P59A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
1 | - |
diphosphate | P104A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
1 | - |
diphosphate | P43A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bacillus sp. PS3 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Bacillus sp. PS3 | important for energy metabolism, provides energy for biosynthetic reactions | 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. PS3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus sp. PS3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Bacillus sp. PS3 | 2 phosphate | - |
? | |
diphosphate + H2O | important for energy metabolism, provides energy for biosynthetic reactions | Bacillus sp. PS3 | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | composed of two trimers, hexameric form required for full activity and thermostability, gel filtration | Bacillus sp. PS3 |
trimer | in the absence of Mg2+ | Bacillus sp. PS3 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
diphosphate | P104A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
2.8 | - |
diphosphate | P100A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
19 | - |
diphosphate | P146A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
74.3 | - |
diphosphate | P14A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
236.7 | - |
diphosphate | P116A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
288.7 | - |
diphosphate | P43A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
413.3 | - |
diphosphate | P59A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
744.3 | - |
diphosphate | P69A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
817 | - |
diphosphate | wild type enzyme, pH 8.0, 37°C | Bacillus sp. PS3 | |
1098 | - |
diphosphate | P39A mutant enzyme, pH 8.0, 37°C | Bacillus sp. PS3 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Bacillus sp. PS3 |