Literature summary for 7.1.2.2 extracted from

Vollmar, M.; Schlieper, D.; Winn, M.; Buechner, C.; Groth, G.
Structure of the c14 rotor ring of the proton translocating chloroplast ATP synthase (2009), J. Biol. Chem., 284, 18228-18235.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified native chloroplast enzyme, hanging drop vapour diffusion method, 0.002 ml protein solution containing 10 mg/ml protein is mixed at 15°C with 0.002 ml crystallization buffer containing 30% v/v PEG 400, 100 mM sodium acetate, pH 4.6, 100 mM cadmium chloride, and 100 mM lithium chloride, and 1 mM ADP to stabilize the F1F0 complex, X-ray diffraction structure determination and analysis at 3.8-4.0 A resolution	Spinacia oleracea

Crystallization (Comment)

Organism

purified native chloroplast enzyme, hanging drop vapour diffusion method, 0.002 ml protein solution containing 10 mg/ml protein is mixed at 15°C with 0.002 ml crystallization buffer containing 30% v/v PEG 400, 100 mM sodium acetate, pH 4.6, 100 mM cadmium chloride, and 100 mM lithium chloride, and 1 mM ADP to stabilize the F1F0 complex, X-ray diffraction structure determination and analysis at 3.8-4.0 A resolution

Spinacia oleracea

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Spinacia oleracea	9507	-
thylakoid membrane	-	Spinacia oleracea	42651	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + H+/in	Spinacia oleracea	-	ADP + phosphate + H+/out	-	r

Organism

Organism	UniProt	Comment	Textmining
Spinacia oleracea	P69447	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from thylakoid membranes by ammonium sulfate fractionation, sucrose density centrifugation, and anion exchange chromatography	Spinacia oleracea

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Spinacia oleracea	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + H+/in	-	Spinacia oleracea	ADP + phosphate + H+/out	-	r
additional information	upon deprotonation, the conformation of Glu61 is changed to another rotamer and becomes fully exposed to the periphery of the ring. Reprotonation of Glu61 by a conserved arginine in the adjacent alpha subunit returns the carboxylate to its initial conformation, structure of putative proton-binding site at the conserved carboxylate Glu61, structure comparison, modelling, overview	Spinacia oleracea	?	-	?

Subunits

Subunits	Comment	Organism
More	structure of the c14 rotor ring of the proton translocating chloroplast ATP synthase, overview	Spinacia oleracea

Synonyms

Synonyms	Comment	Organism
chloroplast ATP synthase	-	Spinacia oleracea
proton translocating chloroplast ATP synthase	-	Spinacia oleracea