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Literature summary for 7.1.2.2 extracted from

  • Wakai, S.; Ohmori, A.; Kanao, T.; Sugio, T.; Kamimura, K.
    Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the atp operon (2005), Biosci. Biotechnol. Biochem., 69, 1884-1891.
    View publication on PubMed
Search for more literature for 7.1.2.2

Activating Compound

Activating Compound Comment Organism Structure
N,N'-dicyclohexylcarbodiimide 0.1 mM increases ATPase activity by 20% Acidithiobacillus ferrooxidans
sulfite 50 mM stimulates 4fold Acidithiobacillus ferrooxidans

Cloned(Commentary)

Cloned (Comment) Organism
strain ATCC3020 in Escherichia coli-derived in vitro transcription-translation system Acidithiobacillus ferrooxidans

Inhibitors

Inhibitors Comment Organism Structure
azide 2 mM inhibits 91% ATPase activity Acidithiobacillus ferrooxidans
Cl-
-
 Acidithiobacillus ferrooxidans
Cu2+ 1 mM reduces ATPase activity 99% in the presence of 5 mM MgSO4 Acidithiobacillus ferrooxidans
Mn2+ 1 mM reduces ATPase activity 50% in the presence of 5 mM MgSO4 Acidithiobacillus ferrooxidans
additional information relatively resistant to vanadate, N,N'-dicyclohexylcarbodiimide and nitrate, 1 mM Fe3+, Ca2+ and Na+ do not affect ATPase activity in the presence of 5 mM MgSO4 Acidithiobacillus ferrooxidans
NaCl inhibits ATPase activity Acidithiobacillus ferrooxidans
Ni2+ 1 mM reduces ATPase activity 47% in the presence of 5 mM MgSO4 Acidithiobacillus ferrooxidans
sulfate slightly inhibits Acidithiobacillus ferrooxidans
Zn2+ 1 mM reduces ATPase activity 80% in the presence of 5 mM MgSO4 Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 ATP estimated at pH 8.5 at 40°C Acidithiobacillus ferrooxidans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Acidithiobacillus ferrooxidans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Acidithiobacillus ferrooxidans

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans
-
 strain NASF-1, strain ATCC33020
-

Purification (Commentary)

Purification (Comment) Organism
51fold by chromatographies, purified ATPase does not contain the F0-portion Acidithiobacillus ferrooxidans

Storage Stability

Storage Stability Organism
4°C, Tris-SO4 buffer, pH 8.5, 8 days Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Acidithiobacillus ferrooxidans ADP + phosphate + H+/out
-
 ?
GTP + H2O + H+/in
-
 Acidithiobacillus ferrooxidans GDP + phosphate + H+/out
-
 ?
ITP + H2O + H+/in
-
 Acidithiobacillus ferrooxidans IDP + phosphate + H+/out
-
 ?
additional information no hydrolysis of UTP nor ADP Acidithiobacillus ferrooxidans ?
-
 ?

Subunits

Subunits Comment Organism
More x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 33000, + x * F1delta, 20000, + x * F1epsilon, 18000, SDS-PAGE of strain NASF-1, x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 30000, + x * F1delta, 23000, + x * F1epsilon, 14000, SDS-PAGE of strain ATCC33020, x * F1alpha, 55500 + x * F1beta, 50500, + x * F1gamma, 33100, + x * F1delta, 19200, + x * F1epsilon, 15100, sequence analysis of strain NASF-1 Acidithiobacillus ferrooxidans

Synonyms

Synonyms Comment Organism
F1-ATPase
-
 Acidithiobacillus ferrooxidans
F1F0-ATPase
-
 Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Acidithiobacillus ferrooxidans

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 ATPase activity decreases markedly above or below this range Acidithiobacillus ferrooxidans