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Literature summary for 7.1.2.1 extracted from

  • Merkulova, M.; McKee, M.; Dip, P.V.; Grueber, G.; Marshansky, V.
    N-terminal domain of the V-ATPase a2-subunit displays integral membrane protein properties (2010), Protein Sci., 19, 1850-1862.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.2.1

Cloned(Commentary)

Cloned (Comment) Organism
subunit a2N1-402 is expressed in Escherichia coli BL21(DE3) cells Homo sapiens

General Stability

General Stability Organism
NDSB-256 is required not only during protein refolding but also afterward for stabilization of a2N1-402 protein in the solution Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane a-subunit Homo sapiens 16020
-
vacuole
-
 Homo sapiens 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 recombinant N-terminal cytosolic tail of V-ATPase a2-subunit isoform a2N1-402, NuPAGE Homo sapiens
48500
-
 recombinant N-terminal cytosolic tail of V-ATPase a2-subunit isoform a2N1-402, calculated from amino acid sequence Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y487
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon bead chromatography, HisTrap column chromatography, and Superdex 200 gel filtration Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
best refolding conditions are 1 M NDSB-256, 1 mM dithiothreitol, 100 mM CHES, and pH 9.0 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Homo sapiens ADP + phosphate + H+/out
-
 ?

Synonyms

Synonyms Comment Organism
V-ATPase
-
 Homo sapiens
vacuolar-type H+-ATPase
-
 Homo sapiens