Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion, 200 mM Zn acetate, 100 mM Na acetate, pH 4.5, 19-22% polyethylene glycol 1000, 10 mg/ml enzyme concentration, hexagonal red crystals appear after 3 d at 20°C | Pseudomonas stutzeri |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas stutzeri | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu | 0.96 mol Cu per mol of enzyme | Pseudomonas stutzeri | |
Fe | 5.3 Fe atoms per enzyme complex | Pseudomonas stutzeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23470 | - |
1 * 58360 + 1 * 34840 + 1 * 23470, MALDI-TOF mass spectrometry | Pseudomonas stutzeri |
26000 | - |
1 * 41000 + 1 * 35000 + 1 * 26000, SDS-PAGE | Pseudomonas stutzeri |
34840 | - |
1 * 58360 + 1 * 34840 + 1 * 23470, MALDI-TOF mass spectrometry | Pseudomonas stutzeri |
35000 | - |
1 * 41000 + 1 * 35000 + 1 * 26000, SDS-PAGE | Pseudomonas stutzeri |
41000 | - |
1 * 41000 + 1 * 35000 + 1 * 26000, SDS-PAGE | Pseudomonas stutzeri |
58360 | - |
1 * 58360 + 1 * 34840 + 1 * 23470, MALDI-TOF mass spectrometry | Pseudomonas stutzeri |
114000 | - |
analytical ultracentrifugation | Pseudomonas stutzeri |
190000 | - |
gel filtration | Pseudomonas stutzeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl, Cu2+-immobilized affinity chromatography | Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + O2 | artificial electron donor: ascorbate/N,N,N',N'-tetramethyl-p-phenylenediamine | Pseudomonas stutzeri | ferricytochrome c + H2O | - |
? | |
N,N,N',N'-tetramethyl-4-phenylendiamine + O2 + H+ | - |
Pseudomonas stutzeri | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 58360 + 1 * 34840 + 1 * 23470, MALDI-TOF mass spectrometry | Pseudomonas stutzeri |
trimer | 1 * 41000 + 1 * 35000 + 1 * 26000, SDS-PAGE | Pseudomonas stutzeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
180 | - |
N,N,N',N'-tetramethyl-4-phenylendiamine | - |
Pseudomonas stutzeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme b | non-covalently bound | Pseudomonas stutzeri | |
heme c | non-covalently bound | Pseudomonas stutzeri |