Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phospholipid | activation | Thermus thermophilus | |
Triton X-100 | 0.5%, 10% activation | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Thermus thermophilus | |
Tween 20 | 0.5%, 40% inhibition | Thermus thermophilus | |
Tween 80 | 0.5% 60% inhibition | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
ferrocytochrome c | Thermus thermophilus cytochrome c | Thermus thermophilus | |
0.041 | - |
ferrocytochrome c | horse cytochrome c | Thermus thermophilus | |
0.074 | - |
ferrocytochrome c | Candida krusei cytochrome c | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu | - |
Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 71000 + 1 * 34000, subunit I shows anomalous behaviour on SDS-PAGE, Ferguson plot | Thermus thermophilus |
71000 | - |
1 * 71000 + 1 * 34000, subunit I shows anomalous behaviour on SDS-PAGE, Ferguson plot | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Renatured (Comment) | Organism |
---|---|
reconstitution into phospholipid vesicles, enzyme acts as proton pump | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + O2 | Candida krusei ferrocytochrome c | Thermus thermophilus | ferricytochrome c + H2O | - |
? | |
ferrocytochrome c + O2 + H+ | horse cytochrome c | Thermus thermophilus | ferricytochrome c + H2O | - |
r | |
ferrocytochrome c + O2 + H+ | Thermus thermophilus cytochrome c | Thermus thermophilus | ferricytochrome c + H2O | - |
r | |
ferrocytochrome c + O2 + H+ | Candida krusei cytochrome c | Thermus thermophilus | ferricytochrome c + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 71000 + 1 * 34000, subunit I shows anomalous behaviour on SDS-PAGE, Ferguson plot | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.88 | - |
ferrocytochrome c | horse cytochrome c | Thermus thermophilus | |
8.33 | - |
ferrocytochrome c | Candida krusei cytochrome c | Thermus thermophilus | |
53 | - |
ferrocytochrome c | Thermus thermophilus cytochrome c | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme a | - |
Thermus thermophilus |