Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Tween 80 | - |
Bacillus subtilis |
General Stability | Organism |
---|---|
ultrafiltration causes inactivation | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 0.08 mM, 50% inhibition | Bacillus subtilis | |
CN- | 0.001 mM, 50% inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme exhibits positive cooperativity at low ionic strength, increasing the KCl concentration to 25 mM causes loss of cooperativity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE | Bacillus subtilis |
37000 | - |
1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE | Bacillus subtilis |
57000 | - |
1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE | Bacillus subtilis |
290000 | 315000 | enzyme associated with detergents | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
method 1: cytochrome c affinity chromatography, ion-exchange, method 2: ammonium sulfate, anion exchange, gel filtration | Bacillus subtilis |
Renatured (Comment) | Organism |
---|---|
reconstitution | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + O2 | horse ferrocytochrome c | Bacillus subtilis | ferricytochrome c + H2O | - |
? | |
ferrocytochrome c + O2 | artificial electron donor: ascorbate/hexaamine ruthenium | Bacillus subtilis | ferricytochrome c + H2O | - |
? | |
ferrocytochrome c + O2 | artificial electron donor: ascorbate/N,N,N',N'-tetramethyl-p-phenylenediamine | Bacillus subtilis | ferricytochrome c + H2O | - |
? | |
ferrocytochrome c + O2 + H+ | - |
Bacillus subtilis | ferricytochrome c + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | 12 | ferrocytochrome c | in the presence of 0.1% dodecyl-beta-D-maltoside | Bacillus subtilis |