Cloned (Comment) | Organism |
---|---|
expression of His-tagged NDH2 in Escherichia coli | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition: dibenziodolium chloride, diphenyliodonium chloride, 1-hydroxy-2-dodecyl-4(1H)quinolone, atovaquone, antimycin A, rotenone, flavone, artemisinin | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
ubiquinone-1 | pH 8.0, 25°C | Plasmodium falciparum | |
0.0046 | - |
oxidized dichlorophenolindophenol | pH 8.0, 25°C | Plasmodium falciparum | |
0.104 | - |
ubiquinone-0 | pH 8.0, 25°C | Plasmodium falciparum | |
0.239 | - |
menadione | pH 8.0, 25°C | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | signal shape and g values of cluster N4 [4Fe(G)C], are particularly sensitive to the changes of the environment around N4 | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged pfNDH2 | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with coenzyme Q4 and coenzyme QD | Plasmodium falciparum | ? | - |
? | |
NADH + H+ + menadione | - |
Plasmodium falciparum | NAD+ + menadiol | - |
? | |
NADH + H+ + oxidized dichlorophenolindophenol | - |
Plasmodium falciparum | NAD+ + reduced dichlorophenolindophenol | - |
? | |
NADH + H+ + ubiquinone-0 | prefers ubiquinol-0 as an acceptor substrate, and can also use the artificial electron acceptors, menadione and dichlorophenolindophenol | Plasmodium falciparum | NAD+ + ubiquinol-0 | - |
? | |
NADH + H+ + ubiquinone-1 | - |
Plasmodium falciparum | NAD+ + ubiquinol-1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NDH2 | - |
Plasmodium falciparum |
PfNdh2 | - |
Plasmodium falciparum |
type II NADH dehydrogenase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
ubiquinone-1 | pH 8.0, 25°C | Plasmodium falciparum | |
0.032 | - |
oxidized dichlorophenolindophenol | pH 8.0, 25°C | Plasmodium falciparum | |
0.077 | - |
menadione | pH 8.0, 25°C | Plasmodium falciparum | |
0.33 | - |
ubiquinone-0 | pH 8.0, 25°C | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 50% of maximal activity, pH 10.0: about 50% of maximal activity | Plasmodium falciparum |