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Literature summary for 7.1.1.2 extracted from

  • Kervinen, M.; Hinttala, R.; Helander, H.M.; Kurki, S.; Uusimaa, J.; Finel, M.; Majamaa, K.; Hassinen, I.E.
    The MELAS mutations 3946 and 3949 perturb the critical structure in a conserved loop of the ND1 subunit of mitochondrial complex I (2006), Hum. Mol. Genet., 15, 2543-2552.
    View publication on PubMed
Search for more literature for 7.1.1.2

Protein Variants

Protein Variants Comment Organism
D213E reduced activity Escherichia coli
D213N reduced activity Escherichia coli
E214K inactive mutant Escherichia coli
E216A reduced activity Escherichia coli
E228D reduced activity Escherichia coli
E228K reduced activity Escherichia coli
E228Q reduced activity Escherichia coli
E234D mutant with slightly reduced activity compared to the wild type enzyme Paracoccus denitrificans
E234Q mutant with strongly reduced activity compared to the wild type enzyme Paracoccus denitrificans
H210F reduced activity Escherichia coli
H210T reduced activity Escherichia coli
R209F reduced activity Escherichia coli
R209H reduced activity Escherichia coli
R209K reduced activity Escherichia coli
V206E reduced activity Escherichia coli
Y229H 30% lower activity than wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
vanillylnonanamide 0.4 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 decylubiquinone mutant enzyme V206E Escherichia coli
2 3 decylubiquinone mutant enzyme Y229H Escherichia coli
25
-
 decylubiquinone mutant enzyme H210T Escherichia coli
33
-
 decylubiquinone mutant enzyme D213E Escherichia coli
34
-
 decylubiquinone mutant enzyme D213N Escherichia coli
37
-
 decylubiquinone mutant enzyme R209F Escherichia coli
40
-
 decylubiquinone mutant enzyme E228D Escherichia coli
43
-
 decylubiquinone wild type enzyme Escherichia coli
43
-
 decylubiquinone mutant enzyme E216A Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Escherichia coli 5739
-

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli GV102
-
-
-
Paracoccus denitrificans
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0098
-
 mutant enzyme E234Q, using ubiquinone-1 as substrate Paracoccus denitrificans
0.15
-
 mutant enzyme E234Q, using hexammineruthenium-(III)-chloride as substrate Paracoccus denitrificans
0.161
-
 wild type enzyme, using ubiquinone-1 as substrate Paracoccus denitrificans
0.163
-
 mutant enzyme E228Q, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.175
-
 mutant enzyme E234D, using ubiquinone-1 as substrate Paracoccus denitrificans
0.211
-
 mutant enzyme R209H, using NADH as substrate Escherichia coli
0.213
-
 mutant enzyme E228K, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.229
-
 mutant enzyme R209F, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.245
-
 mutant enzyme D213E, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.249
-
 mutant enzyme R209H, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.256
-
 mutant enzyme R209F, using NADH as substrate Escherichia coli
0.259
-
 mutant enzyme V206E, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.265
-
 mutant enzyme R209K, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.268
-
 mutant enzyme H210T, using NADH as substrate Escherichia coli
0.268
-
 mutant enzyme R209K, using NADH as substrate Escherichia coli
0.274
-
 mutant enzyme E228K, using NADH as substrate Escherichia coli
0.274
-
 mutant enzyme Y229H, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.305
-
 mutant enzyme E228D, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.31
-
 mutant enzyme H210F, using NADH as substrate Escherichia coli
0.313
-
 mutant enzyme V206E, using NADH as substrate Escherichia coli
0.316
-
 mutant enzyme H210T, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.32
-
 mutant enzyme E228Q, using NADH as substrate Escherichia coli
0.324
-
 mutant enzyme D213E, using NADH as substrate Escherichia coli
0.329
-
 mutant enzyme H210F, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.341
-
 mutant enzyme D213N, using NADH as substrate Escherichia coli
0.348
-
 mutant enzyme Y229H, using NADH as substrate Escherichia coli
0.354
-
 mutant enzyme E216A, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.368
-
 mutant enzyme D213N, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
0.424
-
 mutant enzyme E228D, using NADH as substrate Escherichia coli
0.442
-
 mutant enzyme E216A, using NADH as substrate Escherichia coli
0.63
-
 wild type enzyme, using NADH as substrate Escherichia coli
0.8
-
 wild type enzyme, using hexammineruthenium-(III)-chloride as substrate Escherichia coli
1.144
-
 wild type enzyme, using hexammineruthenium-(III)-chloride as substrate Paracoccus denitrificans
1.34
-
 mutant enzyme E234D, using hexammineruthenium-(III)-chloride as substrate Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + decylubiquinone
-
 Paracoccus denitrificans NAD+ + decylubiquinol
-
 ?
NADH + decylubiquinone
-
 Escherichia coli NAD+ + decylubiquinol
-
 ?
NADH + decylubiquinone
-
 Escherichia coli GV102 NAD+ + decylubiquinol
-
 ?
NADH + hexammineruthenium-(III)-chloride
-
 Paracoccus denitrificans NAD+ + ?
-
 ?
NADH + hexammineruthenium-(III)-chloride
-
 Escherichia coli NAD+ + ?
-
 ?
NADH + hexammineruthenium-(III)-chloride
-
 Escherichia coli GV102 NAD+ + ?
-
 ?
NADH + ubiquinone
-
 Escherichia coli NAD+ + ubiquinol
-
 ?
NADH + ubiquinone
-
 Escherichia coli GV102 NAD+ + ubiquinol
-
 ?
NADH + ubiquinone-1
-
 Paracoccus denitrificans NAD+ + ubiquinol-1
-
 ?

Synonyms

Synonyms Comment Organism
complex I in mitochondria Paracoccus denitrificans
complex I in mitochondria Escherichia coli
ND1
-
 Paracoccus denitrificans
ND1
-
 Escherichia coli
NDH-1 in bacteria Paracoccus denitrificans
NDH-1 in bacteria Escherichia coli
proton-pumping NADH-ubiquinone oxidoreductase
-
 Paracoccus denitrificans
proton-pumping NADH-ubiquinone oxidoreductase
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Paracoccus denitrificans
NADH
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
30
-
 vanillylnonanamide mutant enzyme D213E Escherichia coli
40
-
 vanillylnonanamide wild type enzyme Escherichia coli
41
-
 vanillylnonanamide mutant enzyme D213N Escherichia coli
43
-
 vanillylnonanamide mutant enzyme E228D Escherichia coli