Crystallization (Comment) | Organism |
---|---|
using 30% (w/v) PEG 400 in 0.1 M MES pH 6.5 with 0.1-0.4 M magnesium nitrate and 1 to 2.5% benzamidine hydrochloride | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
T214A | the mutant shows 5% or less of wild type activity | Thermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Thermus thermophilus | 16020 | - |
mitochondrion | - |
Mus musculus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+[side 1] | Mus musculus | - |
NADP+ + NADH + H+[side 2] | - |
? | |
NADPH + NAD+ + H+[side 1] | Thermus thermophilus | - |
NADP+ + NADH + H+[side 2] | - |
? | |
NADPH + NAD+ + H+[side 1] | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
NADP+ + NADH + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Thermus thermophilus | Q72GS0 | subunit beta | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72GS0 | subunit beta | - |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+[side 1] | - |
Mus musculus | NADP+ + NADH + H+[side 2] | - |
? | |
NADPH + NAD+ + H+[side 1] | - |
Thermus thermophilus | NADP+ + NADH + H+[side 2] | - |
? | |
NADPH + NAD+ + H+[side 1] | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | NADP+ + NADH + H+[side 2] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nicotinamide nucleotide transhydrogenase | - |
Mus musculus |
nicotinamide nucleotide transhydrogenase | - |
Thermus thermophilus |
NNT | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme couples electron movement between the NADP/NADPH and NAD/NADH pools to the protonmotive force across the mitochondrial inner membrane | Mus musculus |