Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
expressed in Escherichia coli as cysteine free variant | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A246C | reverse activity stronger affected than cyclic activity | Escherichia coli |
A253C | reverse activity stronger affected than cyclic activity | Escherichia coli |
D238C | reverse activity stronger affected than cyclic activity | Escherichia coli |
G245C | 24% of reverse activity | Escherichia coli |
G245L | 52% of cyclic activity | Escherichia coli |
G245L | the mutation leads to a general inhibition of all enzyme activities | Escherichia coli |
G249C | 40% of reverse activity | Escherichia coli |
G249L | 48% of cyclic activity | Escherichia coli |
G249L | 70% of reverse activity | Escherichia coli |
G249L | the mutation leads to a general inhibition of all enzyme activities | Escherichia coli |
G252C | less than 5% of reverse activity | Escherichia coli |
G252L | 13% of cyclic activity | Escherichia coli |
G252L | the mutation leads to a general inhibition of all enzyme activities | Escherichia coli |
I258C | reverse activity stronger affected than cyclic activity | Escherichia coli |
L240C | reverse activity stronger affected than cyclic activity | Escherichia coli |
L241C | reverse activity stronger affected than cyclic activity | Escherichia coli |
L254C | reverse activity stronger affected than cyclic activity | Escherichia coli |
L255C | reverse activity stronger affected than cyclic activity | Escherichia coli |
M259C | 21% of reverse activity, 215% of cyclic activity | Escherichia coli |
N238C | reverse activity stronger affected than cyclic activity | Escherichia coli |
S250C | strongly increased reverse and cyclic activity | Escherichia coli |
S250C | the mutation leads to enhanced activities of all enzyme activities | Escherichia coli |
S251C | strongly increased reverse and cyclic activity | Escherichia coli |
S251C | the mutation leads to enhanced activities of all enzyme activities | Escherichia coli |
S256C | the mutation leads to enhanced activities of all enzyme activities | Escherichia coli |
T244C | reverse activity stronger affected than cyclic activity | Escherichia coli |
V243C | reverse activity stronger affected than cyclic activity | Escherichia coli |
V248C | reverse activity stronger affected than cyclic activity | Escherichia coli |
Y257C | reverse activity stronger affected than cyclic activity | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + NADP+ | Escherichia coli | links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane | NAD+ + NADPH | - |
r | |
NADPH + NAD+ + H+[side 1] | Escherichia coli | - |
NADP+ + NADH + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
recombinant protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + NADP+ | - |
Escherichia coli | NAD+ + NADPH | - |
r | |
NADH + NADP+ | links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane | Escherichia coli | NAD+ + NADPH | - |
r | |
NADPH + NAD+ + H+[side 1] | - |
Escherichia coli | NADP+ + NADH + H+[side 2] | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | monomer composed of three domians: dI, dII, and dIII | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
proton-translocating nicotinamide nucleotide transhydrogenase | - |
Escherichia coli |
proton-translocating transhydrogenase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Escherichia coli | |
NAD(P)H | - |
Escherichia coli |