Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.7 extracted from

  • Kim, J.H.; Lee, K.K.; Sun, Y.; Seo, G.J.; Cho, S.S.; Kwon, S.H.; Kwon, S.T.
    Broad nucleotide cofactor specificity of DNA ligase from the hyperthermophilic crenarchaeon Hyperthermus butylicus and its evolutionary significance (2013), Extremophiles, 17, 515-522.
    View publication on PubMed
Search for more literature for 6.5.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed under control of the T7lac promoter of pTARG in Escherichia coli BL21-Codon-Plus(DE3)-RIL Hyperthermus butylicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the activity is highly dependent on MgCl2 or MnCl2 with maximal activity above 5 mM MgCl2 and 2 mM MnCl2 Hyperthermus butylicus
Mn2+ the activity is highly dependent on MgCl2 or MnCl2 with maximal activity above 5 mM MgCl2 and 2 mM MnCl2 Hyperthermus butylicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 x * 68000, SDS-PAGE Hyperthermus butylicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Hyperthermus butylicus the multiple cofactor specificity of the the enzyme may possibly be attributed to the ease of ATP decomposition at the high temperatures at which hyperthermophiles grow AMP + phosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Hyperthermus butylicus the multiple cofactor specificity of the the enzyme may possibly be attributed to the ease of ATP decomposition at the high temperatures at which hyperthermophiles grow AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Hyperthermus butylicus DSM Z 5456 the multiple cofactor specificity of the the enzyme may possibly be attributed to the ease of ATP decomposition at the high temperatures at which hyperthermophiles grow AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Hyperthermus butylicus the multiple cofactor specificity of the the enzyme may possibly be attributed to the ease of ATP decomposition at the high temperatures at which hyperthermophiles grow GMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Hyperthermus butylicus DSM Z 5456 the multiple cofactor specificity of the the enzyme may possibly be attributed to the ease of ATP decomposition at the high temperatures at which hyperthermophiles grow GMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Organism

Organism UniProt Comment Textmining
Hyperthermus butylicus
-
-
-
Hyperthermus butylicus DSM Z 5456
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hyperthermus butylicus

Reaction

Reaction Comment Organism Reaction ID
ADP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + phosphate (2) Hyperthermus butylicus
a
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate (1) Hyperthermus butylicus
a
GTP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + GMP + diphosphate (3) Hyperthermus butylicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m the multiple cofactor specificity of the the enzyme may possibly be attributed to the ease of ATP decomposition at the high temperatures at which hyperthermophiles grow Hyperthermus butylicus AMP + phosphate + (deoxyribonucleotide)n+m
-
 ?
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m 95% nick-closing activity when compared with ATP Hyperthermus butylicus AMP + phosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Hyperthermus butylicus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m the multiple cofactor specificity of the the enzyme may possibly be attributed to the ease of ATP decomposition at the high temperatures at which hyperthermophiles grow Hyperthermus butylicus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Hyperthermus butylicus DSM Z 5456 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m the multiple cofactor specificity of the the enzyme may possibly be attributed to the ease of ATP decomposition at the high temperatures at which hyperthermophiles grow Hyperthermus butylicus DSM Z 5456 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m the multiple cofactor specificity of the the enzyme may possibly be attributed to the ease of ATP decomposition at the high temperatures at which hyperthermophiles grow Hyperthermus butylicus GMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m 86% nick-closing activity when compared with ATP Hyperthermus butylicus GMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m the multiple cofactor specificity of the the enzyme may possibly be attributed to the ease of ATP decomposition at the high temperatures at which hyperthermophiles grow Hyperthermus butylicus DSM Z 5456 GMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m 86% nick-closing activity when compared with ATP Hyperthermus butylicus DSM Z 5456 GMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
additional information no activity with NAD+, CTP or TTP Hyperthermus butylicus ?
-
 ?
additional information no activity with NAD+, CTP or TTP Hyperthermus butylicus DSM Z 5456 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 68000, SDS-PAGE Hyperthermus butylicus

Synonyms

Synonyms Comment Organism
Hbu DNA ligase
-
 Hyperthermus butylicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Hyperthermus butylicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 80 60°C: about 55% of maximal activity, 80°C: about 60% of maximal activity Hyperthermus butylicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
94
-
 2.3 h, about 50% loss of activity Hyperthermus butylicus
99
-
 half-life: 1.7 h Hyperthermus butylicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in Tris-HCl buffer Hyperthermus butylicus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 pH 6.5: about 90% of maximal activity, 9.0: about 95% of maximal activity Hyperthermus butylicus