Cloned (Comment) | Organism |
---|---|
the catalytic domain of human LIG1 (residues 232-919) is expressed in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | EDTA rapidly inactivates all LIG1-catalyzed reactions | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
ATP | at 30 mM Mg2+, pH and temperature not specified in the publication | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | LIG1 requires multiple Mg2+ ions for catalysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Q-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | synthetic 28-mer oligonucleotide duplex that contains a nick with a 3'-hydroxyl and a 5'-phosphate, more than 95% of the nicked DNA is ligated within 2 s | Homo sapiens | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
additional information | LIG1 catalyzes the ligation of single-strand breaks to complete DNA replication and repair. The energy of ATP is used to form a new phosphodiester bond in DNA via a reaction mechanism that involves three distinct chemical steps: enzyme adenylylation, adenylyl transfer to DNA, and nick sealing | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA ligase I | - |
Homo sapiens |
LIG1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
ATP | at 30 mM Mg2+, pH and temperature not specified in the publication | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | the efficiency of ligation falls from near 100% under optimal conditions with 1 mM Mg2+ and 10 mM ATP to 60% with 1 mM Mg2+ and 2 mM ATP | Homo sapiens |