Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanothermobacter thermautotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
D253A | mutant DNA ligase reacts with ATP to form the covalent intermediate, but is unable to catalyze the full ligation reaction | Methanothermobacter thermautotrophicus |
K251A | no DNA ligase-adenylate formation and no nick-joining | Methanothermobacter thermautotrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | 10 mM, 60% inhibition | Methanothermobacter thermautotrophicus | |
KCl | 50 mM, 70% inhibition | Methanothermobacter thermautotrophicus | |
NaCl | 50 mM, 75% inhibition, 80 mM, 90% inhibition | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
ATP | 60°C, pH 7.5 | Methanothermobacter thermautotrophicus | |
0.0016 | - |
dATP | 60°C, pH 7.5 | Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, divalent cation required for activity | Methanothermobacter thermautotrophicus | |
Mg2+ | 10 mM, divalent cation required for activity | Methanothermobacter thermautotrophicus | |
Mn2+ | 10 mM, divalent cation required for activity | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
zonal velocity sedimentation | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Methanothermobacter thermautotrophicus | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | Q50566 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant Mth ligase | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | strandjoining on a singly nicked DNA in the presence of a divalent cation and ATP | Methanothermobacter thermautotrophicus | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | strandjoining on a singly nicked DNA in the presence of a divalent cation and dATP, approx. 10% of activity with ATP | Methanothermobacter thermautotrophicus | dAMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 68000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Synonyms | Comment | Organism |
---|---|---|
Mth ligase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 80 | almost no activity at 22°C, approx. 25% of maximal activity at 40°C and 70°C | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.8 | - |
Methanothermobacter thermautotrophicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | approx. 20% of maximal activity at pH 6.5, approx. 90% of maximal activity at pH 9.0 | Methanothermobacter thermautotrophicus |