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Literature summary for 6.4.1.4 extracted from

  • McKean, A.L.; Ke, J.; Song, J.; Che, P.; Achenbach, S.; Nikolau, B.J.; Wurtele, E.S.
    Molecular characterization of the non-biotin-containing subunit of 3-methylcrotonyl-CoA carboxylase (2000), J. Biol. Chem., 275, 5582-5590.
    View publication on PubMed
Search for more literature for 6.4.1.4

Application

Application Comment Organism
agriculture MCCase is of significance in comprehending how the mevalonate shunt can divert carbon away from the biosynthesis of isoprenoids, such as cholesterol, which has major implications in the prevention of vascular degenerate diseases Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
cDNA cloned and expressed in Escherichia coli Arabidopsis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 85000 + x * 60000, heteromeric enzyme composed of biotin-containing MCC-A and non-biotin-containing MCC-B subunits Arabidopsis thaliana
85000
-
 x * 85000 + x * 60000, heteromeric enzyme composed of biotin-containing MCC-A and non-biotin-containing MCC-B subunits Arabidopsis thaliana
900000
-
 nondenaturing PAGE, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3-methylcrotonyl-CoA + HCO3- Arabidopsis thaliana pivotal reaction required for both leucine catabolism and isoprenoid metabolism ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LDD8 ecotype Columbia
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-methylcrotonyl-CoA + HCO3-
-
 Arabidopsis thaliana ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir
ATP + 3-methylcrotonyl-CoA + HCO3- pivotal reaction required for both leucine catabolism and isoprenoid metabolism Arabidopsis thaliana ADP + phosphate + 3-methylglutaconyl-CoA
-
 ir

Subunits

Subunits Comment Organism
polymer x * 85000 + x * 60000, heteromeric enzyme composed of biotin-containing MCC-A and non-biotin-containing MCC-B subunits Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
3-methylcrotonyl-CoA carboxylase
-
 Arabidopsis thaliana
3-methylcrotonyl-CoA:carbon-dioxide ligase (ADP-forming)
-
 Arabidopsis thaliana
MCCase
-
 Arabidopsis thaliana