Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | dependent, half maximal activation at 0.014 mM | Mycolicibacterium smegmatis | |
butyryl-CoA | can replace acetyl-CoA with little efficiency | Mycolicibacterium smegmatis | |
propionyl-CoA | can replace acetyl-CoA with little efficiency | Mycolicibacterium smegmatis |
Cloned (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Mycolicibacterium smegmatis | |
ATP | - |
Mycolicibacterium smegmatis | |
Co2+ | at excess concentrations | Mycolicibacterium smegmatis | |
L-aspartate | 47% inhibition at 10 mM | Mycolicibacterium smegmatis | |
Mg2+ | at excess concentrations | Mycolicibacterium smegmatis | |
Mn2+ | at excess concentrations | Mycolicibacterium smegmatis | |
additional information | no inhibitors: glutamate and alpha-ketoglutarate | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ATP | 37°C and 45°C, pH 8, Mg2+ concentration higher than ATP concentration | Mycolicibacterium smegmatis | |
0.3 | - |
pyruvate | 37°C, pH 8 | Mycolicibacterium smegmatis | |
0.45 | - |
pyruvate | 45°C, pH 8 | Mycolicibacterium smegmatis | |
1.26 | - |
ATP | 37°C, pH 8, Mg2+ concentration equal to that of ATP | Mycolicibacterium smegmatis | |
3 | - |
HCO3- | 37°C, pH 8 | Mycolicibacterium smegmatis | |
4.6 | - |
HCO3- | 45°C, pH 8 | Mycolicibacterium smegmatis | |
9.2 | - |
ATP | 45°C, pH 8, Mg2+ concentration equal to that of ATP | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mg2+ with little efficiency | Mycolicibacterium smegmatis | |
Mg2+ | requirement | Mycolicibacterium smegmatis | |
Mn2+ | can replace Mg2+ with little efficiency | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
x * 120000, SDS-PAGE | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | Mycolicibacterium smegmatis | anaplerotic enzyme | ADP + phosphate + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150 | - |
- |
Mycolicibacterium smegmatis |
Storage Stability | Organism |
---|---|
-70°C, 90% activity after 1 month in the presence of 10% inositol, 30% loss of activity after 24 h without inositol | Mycolicibacterium smegmatis |
4°C, 90% activity after 1 month in the presence of 10% inositol, 30% loss of activity after 24 h without inositol | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | - |
Mycolicibacterium smegmatis | ADP + phosphate + oxaloacetate | - |
? | |
ATP + pyruvate + HCO3- | anaplerotic enzyme | Mycolicibacterium smegmatis | ADP + phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 120000, SDS-PAGE | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
PC | - |
Mycolicibacterium smegmatis |
PYC | - |
Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | - |
Mycolicibacterium smegmatis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.24 | - |
ATP | 45°C, pH 8 | Mycolicibacterium smegmatis |