Cloned (Comment) | Organism |
---|---|
subcloning of each of the subunits into the pET-28b vector, expression in Escherichia coli | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE | Methanocaldococcus jannaschii |
71000 | - |
x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | cross-talk between domain N and C is required for product release from subunit C | Methanocaldococcus jannaschii | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000 (subunit N) + x * 71000 (subunit C), SDS-PAGE | Methanocaldococcus jannaschii |