Application | Comment | Organism |
---|---|---|
medicine | erythrocyte NAD synthetase is activated by lead, and the activity is a sensitive indicator of lead exposure in human | Homo sapiens |
General Stability | Organism |
---|---|
EDTA and KCl increase stability substantially | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pb2+ | - |
Homo sapiens | |
Zn2+ | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.108 | - |
deamido-NAD+ | - |
Homo sapiens | |
0.154 | - |
ATP | - |
Homo sapiens | |
2.17 | - |
Gln | - |
Homo sapiens | |
64.2 | - |
NH4+ | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required | Homo sapiens | |
K+ | Km: 8.32 mM | Homo sapiens | |
Mg2+ | required | Homo sapiens | |
Mg2+ | Km: 1.36 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
483000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.017 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, 24 h, complete loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + L-Gln | - |
Homo sapiens | AMP + diphosphate + NAD+ + L-Glu | - |
? | |
ATP + deamido-NAD+ + NH4+ | - |
Homo sapiens | AMP + diphosphate + NAD+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.4 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 6.0: about 75% of maximal activity, 8.0: about 55% of maximal activity | Homo sapiens |