Cloned (Comment) | Organism |
---|---|
cDNA | Dictyostelium discoideum |
gene ade2 | Schizosaccharomyces pombe |
mouse muscle gene AdSS1 and mouse nonmuscle gene AdSS2 | Mus musculus |
purA gene | Acidithiobacillus ferrooxidans |
purA gene | Haemophilus influenzae |
purA gene | Bacillus subtilis |
purA gene overexpressed in strain H1238 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R143K | site-directed mutagenesis | Escherichia coli |
R143L | site-directed mutagenesis | Escherichia coli |
R303L | site-directed mutagenesis | Escherichia coli |
R304L | site-directed mutagenesis | Escherichia coli |
R305L | site-directed mutagenesis | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hadacidin | - |
Escherichia coli | |
Hadacidin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
GTP | - |
Escherichia coli | |
0.01 | - |
L-aspartate | - |
Escherichia coli | |
0.02 | - |
IMP | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | absolute requirement for divalent metal ions | Escherichia coli | |
Ca2+ | absolute requirement for divalent metal ions | Escherichia coli | |
Co2+ | absolute requirement for divalent metal ions | Escherichia coli | |
Cu2+ | absolute requirement for divalent metal ions | Escherichia coli | |
Mg2+ | absolute requirement for divalent metal ions, best activator | Escherichia coli | |
Mn2+ | absolute requirement for divalent metal ions | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46600 | - |
2 * 46600 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-aspartate | Acidithiobacillus ferrooxidans | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Pyrococcus sp. | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Haemophilus influenzae | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Bacillus subtilis | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Mus musculus | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Escherichia coli | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Homo sapiens | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Rattus norvegicus | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Triticum aestivum | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Oryctolagus cuniculus | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Zea mays | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Arabidopsis thaliana | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Dictyostelium discoideum | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Schizosaccharomyces pombe | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Leishmania donovani | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Azotobacter vinelandii | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Trypanosoma cruzi | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Methanocaldococcus jannaschii | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | Pyrococcus sp. ST700 | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
- |
- |
Arabidopsis thaliana | - |
- |
- |
Azotobacter vinelandii | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Dictyostelium discoideum | - |
- |
- |
Escherichia coli | - |
- |
- |
Haemophilus influenzae | - |
- |
- |
Homo sapiens | - |
human | - |
Leishmania donovani | - |
- |
- |
Methanocaldococcus jannaschii | - |
- |
- |
Mus musculus | - |
mouse | - |
Oryctolagus cuniculus | - |
rabbit | - |
Pyrococcus sp. | - |
- |
- |
Pyrococcus sp. ST700 | - |
- |
- |
Rattus norvegicus | - |
rat | - |
Schizosaccharomyces pombe | - |
- |
- |
Triticum aestivum | - |
wheat | - |
Trypanosoma cruzi | - |
- |
- |
Zea mays | - |
maize | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Oryctolagus cuniculus |
- |
Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-aspartate | - |
Acidithiobacillus ferrooxidans | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Pyrococcus sp. | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Haemophilus influenzae | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Bacillus subtilis | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Mus musculus | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Homo sapiens | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Rattus norvegicus | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Triticum aestivum | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Oryctolagus cuniculus | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Zea mays | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Arabidopsis thaliana | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Dictyostelium discoideum | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Schizosaccharomyces pombe | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Leishmania donovani | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Azotobacter vinelandii | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Trypanosoma cruzi | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Methanocaldococcus jannaschii | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Acidithiobacillus ferrooxidans | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Pyrococcus sp. | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Haemophilus influenzae | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Bacillus subtilis | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Mus musculus | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Homo sapiens | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Rattus norvegicus | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Triticum aestivum | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Oryctolagus cuniculus | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Zea mays | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Arabidopsis thaliana | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Dictyostelium discoideum | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Schizosaccharomyces pombe | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Leishmania donovani | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Azotobacter vinelandii | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Trypanosoma cruzi | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Methanocaldococcus jannaschii | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | - |
Pyrococcus sp. ST700 | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle | Pyrococcus sp. ST700 | GDP + phosphate + adenylosuccinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 46600 | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
adenosylsuccinate synthetase | - |
Escherichia coli |
adenosylsuccinate synthetase | - |
Rattus norvegicus |
Adenylosuccinate synthetase | - |
Acidithiobacillus ferrooxidans |
Adenylosuccinate synthetase | - |
Pyrococcus sp. |
Adenylosuccinate synthetase | - |
Haemophilus influenzae |
Adenylosuccinate synthetase | - |
Bacillus subtilis |
Adenylosuccinate synthetase | - |
Mus musculus |
Adenylosuccinate synthetase | - |
Homo sapiens |
Adenylosuccinate synthetase | - |
Triticum aestivum |
Adenylosuccinate synthetase | - |
Oryctolagus cuniculus |
Adenylosuccinate synthetase | - |
Zea mays |
Adenylosuccinate synthetase | - |
Arabidopsis thaliana |
Adenylosuccinate synthetase | - |
Dictyostelium discoideum |
Adenylosuccinate synthetase | - |
Schizosaccharomyces pombe |
Adenylosuccinate synthetase | - |
Leishmania donovani |
Adenylosuccinate synthetase | - |
Azotobacter vinelandii |
Adenylosuccinate synthetase | - |
Trypanosoma cruzi |
Adenylosuccinate synthetase | - |
Methanocaldococcus jannaschii |
AMPSase | - |
Acidithiobacillus ferrooxidans |
AMPSase | - |
Pyrococcus sp. |
AMPSase | - |
Haemophilus influenzae |
AMPSase | - |
Bacillus subtilis |
AMPSase | - |
Mus musculus |
AMPSase | - |
Escherichia coli |
AMPSase | - |
Homo sapiens |
AMPSase | - |
Rattus norvegicus |
AMPSase | - |
Triticum aestivum |
AMPSase | - |
Oryctolagus cuniculus |
AMPSase | - |
Zea mays |
AMPSase | - |
Arabidopsis thaliana |
AMPSase | - |
Dictyostelium discoideum |
AMPSase | - |
Schizosaccharomyces pombe |
AMPSase | - |
Leishmania donovani |
AMPSase | - |
Azotobacter vinelandii |
AMPSase | - |
Trypanosoma cruzi |
AMPSase | - |
Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
GTP | - |
Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | acidic isoenzyme | - |
5.9 |
Rattus norvegicus | basic isoenzyme | - |
8.9 |
Mus musculus | most abundant isozyme | - |
8.9 |