Protein Variants | Comment | Organism |
---|---|---|
R147L | mutant R147L shows increased Km for IMP and GTP relative to the wild-type enzyme, Km for Asp exhibits a modest decrease | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phenylglyoxal | GTP or IMP partially protect | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
GTP | wild-type | Escherichia coli | |
0.024 | - |
IMP | wild-type | Escherichia coli | |
0.115 | - |
Asp | mutant R147L | Escherichia coli | |
0.118 | - |
IMP | mutant R147L | Escherichia coli | |
0.133 | - |
GTP | mutant R147L | Escherichia coli | |
0.191 | - |
Asp | wild-type | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant R147L | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-Asp | - |
Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |