Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces pombe | Lithobates catesbeianus |
Protein Variants | Comment | Organism |
---|---|---|
E261Q | no synthesis of carbonyl phosphate in presence of L-glutamine | Lithobates catesbeianus |
K258L | synthesis of carbonyl phosphate in presence of L-glutamine | Lithobates catesbeianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
ATP | wild-type enzyme, comparison to native and mutant enzyme | Lithobates catesbeianus | |
0.39 | - |
ammonia | wild-type enzyme, comparison to native and mutant enzyme | Lithobates catesbeianus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + NH4+ + HCO3- | Homo sapiens | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | Rattus norvegicus | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | Lithobates catesbeianus | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Lithobates catesbeianus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lithobates catesbeianus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate | depending on their physiological role, carbonyl phosphate synthetases use either glutamine or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis, all enzymes contain the structurally equivalent of a triad-type glutamine amidotransferase, GAT, domain | Lithobates catesbeianus | |
2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate | depending on their physiological role, carbonyl phosphate synthetases uses either glutamine or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis, all enzymes contain the structurell equivalent of a triad-type glutamine amidotransferase, GAT, domain | Homo sapiens | |
2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate | depending on their physiological role, carbonyl phosphate synthetasese uses either glutamine or free ammonia as the nitrogen donor for carbamoyl phosphate synthesis, all enzymes contain the structurell equivalent of a triad-type glutamine amidotransferase, GAT, domain | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + NH4+ + HCO3- | - |
Homo sapiens | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Rattus norvegicus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Lithobates catesbeianus | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
ammonia | wild-type enzyme, comparison to native and mutant enzyme | Lithobates catesbeianus | |
2.4 | - |
ATP | wild-type enzyme, comparison to native and mutant enzyme | Lithobates catesbeianus |