Cloned (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 8-12% (w/v) PEG8000 in 0.1 M Tris pH 7.5 or 8.0 and 10% (v/v) glycerol | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
R125A | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Staphylococcus aureus |
R125N | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(3aS,4S,6aR)-4-(5-[1-[3-(naphthalen-2-yloxy)propyl]-1H-1,2,3-triazol-4-yl]pentyl)tetrahydro-1H-thieno[3,4-d]imidazol-2(3H)-one | - |
Staphylococcus aureus | |
(3aS,4S,6aR)-4-(5-[1-[4-(naphthalen-1-yloxy)butyl]-1H-1,2,3-triazol-4-yl]pentyl)tetrahydro-1H-thieno[3,4-d]imidazol-2(3H)-one | - |
Staphylococcus aureus | |
(3aS,4S,6aR)-4-(5-[1-[4-(naphthalen-2-yloxy)butyl]-1H-1,2,3-triazol-4-yl]pentyl)tetrahydro-1H-thieno[3,4-d]imidazol-2(3H)-one | - |
Staphylococcus aureus | |
(3aS,4S,6aR)-4-[-[1-[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]triazol-4-yl]pentyl]-1,3,3a,4,6,6ahexahydrothieno[3,4-d]imidazol-2-one | competitive inhibitor | Staphylococcus aureus | |
(3aS,4S,6aR)-4-[4-[1-[[(3aR,4R,6R,6aR)-4-(6-aminopurin-9-yl)-2,2-dimethyl-3a,4,6,6a-tetrahydrofuro[3,4-d][1,3]dioxol-6-yl]methyl]triazol-4-yl]butyl]-1,3,3a,4,6,6a-hexa hydrothieno[3,4-d]imidazol-2-one | - |
Staphylococcus aureus | |
(3aS,4S,6aR)-4-[5-[1-(4-phenoxybutyl)-1H-1,2,3-triazol-4-yl]pentyl]tetrahydro-1H-thieno[3,4-d]imidazol-2(3H)-one | - |
Staphylococcus aureus | |
(3aS,4S,6aR)-4-[5-[1-[[(3aR,4R,6R,6aR)-4-(6-aminopurin-9-yl)-2,2-dimethyl-3a,4,6,6a-tetrahydrofuro[3,4-d][1,3]dioxol-6-yl]methyl]triazol-4-yl]pentyl]-1,3,3a,4,6,6a-hexa hydrothieno[3,4-d]imidazol-2-one | - |
Staphylococcus aureus | |
(3aS,4S,6aR)-4-[5-[3-[[(3aR,4R,6R,6aR)-4-(6-aminopurin-9-yl)-2,2-dimethyl-3a,4,6,6a-tetrahydrofuro[3,4-d][1,3]dioxol-6-yl]methyl]triazol-4-yl]pentyl]-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-2-one | - |
Staphylococcus aureus | |
(3aS,4S,6aR)-4-[6-[1-[[(3aR,4R,6R,6aR)-4-(6-aminopurin-9-yl)-2,2-dimethyl-3a,4,6,6a-tetrahydrofuro[3,4-d][1,3]dioxol-6-yl]methyl]triazol-4-yl]hexyl]-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-2-one | - |
Staphylococcus aureus | |
2-(4-[5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentyl]-1H-1,2,3-triazol-1-yl)ethyl 2,2-dimethylpropanoate | - |
Staphylococcus aureus | |
5-methyl-3-[3-(4-[5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentyl]-1H-1,2,3-triazol-1-yl)propyl]-1,3-benzoxazol-2(3H)-one | - |
Staphylococcus aureus | |
5-methyl-3-[4-(4-[5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentyl]-1H-1,2,3-triazol-1-yl)butyl]-1,3-benzoxazol-2(3H)-one | competitive inhibitor versus biotin | Staphylococcus aureus | |
biotinol-5'-AMP | - |
Staphylococcus aureus | |
biotinyl-5'-AMP | selective inhibitor | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00101 | - |
biotin | wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.00108 | - |
biotin | mutant enzyme R125A, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.00108 | - |
biotin | mutant enzyme R125N, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.18 | - |
ATP | wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.35 | - |
ATP | mutant enzyme R125N, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.42 | - |
ATP | mutant enzyme R125A, at pH 7.5 and 37°C | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Staphylococcus aureus | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | Staphylococcus aureus ECT-2 | - |
AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus ECT-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Staphylococcus aureus | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? | |
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
Staphylococcus aureus ECT-2 | AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
biotin protein ligase | - |
Staphylococcus aureus |
BPL | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
440 | - |
ATP | mutant enzyme R125A, at pH 7.5 and 37°C | Staphylococcus aureus | |
520 | - |
ATP | mutant enzyme R125N, at pH 7.5 and 37°C | Staphylococcus aureus | |
810 | - |
biotin | mutant enzyme R125A, at pH 7.5 and 37°C | Staphylococcus aureus | |
840 | - |
biotin | mutant enzyme R125N, at pH 7.5 and 37°C | Staphylococcus aureus | |
360000 | - |
ATP | wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus | |
450000 | - |
biotin | wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00009 | - |
5-methyl-3-[4-(4-[5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentyl]-1H-1,2,3-triazol-1-yl)butyl]-1,3-benzoxazol-2(3H)-one | wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.00117 | - |
(3aS,4S,6aR)-4-[-[1-[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]triazol-4-yl]pentyl]-1,3,3a,4,6,6ahexahydrothieno[3,4-d]imidazol-2-one | wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.0017 | - |
(3aS,4S,6aR)-4-(5-[1-[4-(naphthalen-2-yloxy)butyl]-1H-1,2,3-triazol-4-yl]pentyl)tetrahydro-1H-thieno[3,4-d]imidazol-2(3H)-one | wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.01 | - |
2-(4-[5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentyl]-1H-1,2,3-triazol-1-yl)ethyl 2,2-dimethylpropanoate | Ki above 0.01 mM, wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.01 | - |
(3aS,4S,6aR)-4-[5-[1-(4-phenoxybutyl)-1H-1,2,3-triazol-4-yl]pentyl]tetrahydro-1H-thieno[3,4-d]imidazol-2(3H)-one | Ki above 0.01 mM, wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.01 | - |
(3aS,4S,6aR)-4-(5-[1-[4-(naphthalen-1-yloxy)butyl]-1H-1,2,3-triazol-4-yl]pentyl)tetrahydro-1H-thieno[3,4-d]imidazol-2(3H)-one | Ki above 0.01 mM, wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.01 | - |
(3aS,4S,6aR)-4-(5-[1-[3-(naphthalen-2-yloxy)propyl]-1H-1,2,3-triazol-4-yl]pentyl)tetrahydro-1H-thieno[3,4-d]imidazol-2(3H)-one | Ki above 0.01 mM, wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus | |
0.01 | - |
5-methyl-3-[3-(4-[5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentyl]-1H-1,2,3-triazol-1-yl)propyl]-1,3-benzoxazol-2(3H)-one | Ki above 0.01 mM, wild type enzyme, at pH 7.5 and 37°C | Staphylococcus aureus |