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Literature summary for 6.3.4.15 extracted from

  • Chapman-Smith, A.; Mulhern, T.D.; Whelan, F.; Cronan, J.E., Jr.; Wallace, J.C.
    The C-terminal domain of biotin protein ligase from E. coli is required for catalytic activity (2001), Protein Sci., 10, 2608-2617.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.15

Cloned(Commentary)

Cloned (Comment) Organism
expression of BirA from pET-based vector pHBA in Escherichia coli BL21 (lambdaDE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
G115S site-directed mutagenesis Escherichia coli
I272X site-directed mutagenesis Escherichia coli
K122E site-directed mutagenesis Escherichia coli
K277E site-directed mutagenesis Escherichia coli
K283E site-directed mutagenesis Escherichia coli
K321E site-directed mutagenesis Escherichia coli
R182E site-directed mutagenesis Escherichia coli
R212E site-directed mutagenesis Escherichia coli
R213E site-directed mutagenesis Escherichia coli
R317E site-directed mutagenesis Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 biotin mutant K283E Escherichia coli
0.00027
-
 biotin mutant K321E Escherichia coli
0.0003
-
 biotin wild-type enzyme Escherichia coli
0.0006
-
 biotin mutant K277E Escherichia coli
0.0007
-
 biotin mutant R317E Escherichia coli
0.0025
-
 biotin mutant R317E, wild-type BCCP biotin domain Escherichia coli
0.0033
-
 biotin mutant R317E, E147Kmutant BCCP biotin domain Escherichia coli
0.0035
-
 biotin mutant K277E, wild-type BCCP biotin domain Escherichia coli
0.006
-
 biotin mutant K321E, wild-type BCCP biotin domain Escherichia coli
0.007
-
 biotin wild-type enzyme, wild-type BCCP biotin domain Escherichia coli
0.008
-
 biotin mutant K283E, wild-type BCCP biotin domain Escherichia coli
0.009
-
 biotin mutant K277E, E147Kmutant BCCP biotin domain Escherichia coli
0.0112
-
 biotin wild-type enzyme, E147Kmutant BCCP biotin domain Escherichia coli
0.013
-
 biotin mutant K321E, E147Kmutant BCCP biotin domain Escherichia coli
0.015
-
 biotin mutant K283E, E147Kmutant BCCP biotin domain Escherichia coli
0.2
-
 ATP wild-type enzyme Escherichia coli
0.28
-
 ATP mutant K321E Escherichia coli
0.29
-
 ATP mutant K283E Escherichia coli
1.37
-
 ATP mutant K277E Escherichia coli
5.1
-
 ATP mutant R317E Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35300
-
 SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli also functions to repress the biotin biosynthetic operon and synthesizes its own corepressor, biotinyl-5'-AMP, the catalytic intermediate of the biotinylation reaction, catalyzes key reactions in essential metabolic processes, essential for survival ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutants K277E, R317E, and K321E Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine ATP in form of MgATP2- Escherichia coli AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine
-
 ?
biotin + ATP + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] catalyzes the covalent attachment of the biotin prosthetic group to a specific lysine of the biotin carboxyl carrier domain of biotin-dependent carboxylases in a two-step reaction Escherichia coli AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 r
additional information also functions to repress the biotin biosynthetic operon and synthesizes its own corepressor, biotinyl-5'-AMP, the catalytic intermediate of the biotinylation reaction, catalyzes key reactions in essential metabolic processes, essential for survival Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
biotin protein ligase
-
 Escherichia coli
BirA
-
 Escherichia coli
BirA protein
-
 Escherichia coli
Holocarboxylase synthetase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.037
-
 biotin mutant R317E Escherichia coli
0.081
-
 ATP mutant K277E Escherichia coli
0.09
-
 biotin mutant R317E, E147Kmutant BCCP biotin domain Escherichia coli
0.12
-
 biotin mutant R317E, wild-type BCCP biotin domain Escherichia coli
0.17
-
 ATP wild-type enzyme Escherichia coli
0.17
-
 biotin mutant K283E Escherichia coli
0.2
-
 ATP mutant K321E Escherichia coli
0.24
-
 biotin wild-type enzyme, E147Kmutant BCCP biotin domain Escherichia coli
0.25
-
 biotin mutant K277E, E147Kmutant BCCP biotin domain Escherichia coli
0.26
-
 biotin mutant K277E, wild-type BCCP biotin domain Escherichia coli
0.28
-
 ATP mutant K283E Escherichia coli
0.28
-
 biotin mutant K321E Escherichia coli
0.3
-
 biotin wild type enzyme and mutant K277E Escherichia coli
0.32
-
 ATP mutant K321E Escherichia coli
0.44
-
 biotin wild-type enzyme, wild-type BCCP biotin domain Escherichia coli
0.6
-
 biotin mutant K321E, E147Kmutant BCCP biotin domain Escherichia coli
0.8
-
 biotin mutant K321E, wild-type BCCP biotin domain and mutant K283E, E147Kmutant BCCP biotin domain Escherichia coli
0.9
-
 biotin mutant K283E, wild-type BCCP biotin domain Escherichia coli