Inhibitors | Comment | Organism | Structure |
---|---|---|---|
biotin | above 300 mM, noncompetitive substrate inhibitor | Escherichia coli | |
additional information | no substrate inhibition by ATP | Escherichia coli | |
Phosphonoacetate | competitive inhibition versus ATP, noncompetitive versus bicarbonate | Escherichia coli | |
phosphonoacetate linked to the 1'-nitrogen of biotin | reaction intermediate analog, modest inhibition, competitive versus ATP, noncompetitive versus biotin | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ordered kinetic mechanism | Escherichia coli | |
134 | - |
biotin | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + HCO3- | Escherichia coli | long-chain fatty acid synthesis, in vivo biotin is covalently attached to the biotin-carboxyl-carrier protein | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine | kinetic mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + HCO3- | biotin carboxylase component of the multienzyme complex acetyl-CoA carboxylase, also utilizes free biotin as substrate | Escherichia coli | ADP + phosphate + carboxybiotin | - |
? | |
ATP + biotin-carboxyl-carrier protein + HCO3- | long-chain fatty acid synthesis, in vivo biotin is covalently attached to the biotin-carboxyl-carrier protein | Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
ATP + biotin-carboxyl-carrier protein + HCO3- | biotin carboxylase component of the multienzyme complex acetyl-CoA carboxylase, catalyzes the first half-reaction of the reaction catalyzed by acetyl-CoA carboxylase, the ATP-dependent carboxylation of biotin, ordered addition of substrates with ATP binding first followed by bicarbonate and then biotin | Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
Phosphonoacetate | - |
Escherichia coli | |
8 | - |
phosphonoacetate linked to the 1'-nitrogen of biotin | - |
Escherichia coli |